Supplementary Figure 4 : Gene expression analyses in PWS cell lines.

From: The noncoding RNA IPW regulates the imprinted DLK1-DIO3 locus in an induced pluripotent stem cell model of Prader-Willi syndrome

Supplementary Figure 4

(a) Analysis of gene expression by moving average plot along chromosome 14q in PWS parental fibroblasts relative to control fibroblasts. Vertical dashed lines mark the genomic boundaries of the DLK1-DIO3 region. (b) qRT-PCR of the mean relative fold change ± s.d. (three biological replicates each) in MEG3 in mature PWS cells derived from three affected individuals, showing negligible expression levels in patient-derived B cells. N.E., not expressed. (c) Expression analysis of all MEGs represented in the expression arrays in the parental fibroblasts (left panel) and undifferentiated iPSCs (right panel). WT, control.