Supplementary Figure 3 : Analysis of known imprinted genes in PWS-iPSCs.

From: The noncoding RNA IPW regulates the imprinted DLK1-DIO3 locus in an induced pluripotent stem cell model of Prader-Willi syndrome

Supplementary Figure 3

(a) RTL1 expression ratio (in RPKM, logarithmic scale, two biological replicates), demonstrating its marked downregulation in Pg-iPSCs relative to normal PSCs. (b) qRT-PCR of the mean relative fold change ± s.d. (three biological replicates each) of RTL1, MEG3, miR-370 and miR-409 in normal PSCs before and after knockdown of RTL1 (+siRNA). (c) qRT-PCR of the mean relative fold change ± s.d. of miR-371-3 in normal PSCs, Pg-iPSCs and PWS-iPSCs (two biological replicates each), demonstrating comparable expression levels of miR-371-3 in PWS-iPSCs and normal PSCs. (d) Mean expression levels ± s.d. of representative known PEGs in distinct loci. Analyses were conducted on three PWS-iPSC lines (PWS-iPSC-1-A and B and PWS-iPSC-2-A), three Pg-iPSC lines (Pg-iPSC-A-11, 20 and 26) and five control PSCs (two iPSC and three ESC lines). WT-PSCs, normal PSCs.