Supplementary Figure 1 : Characterization of PWS-iPSCs.

From: The noncoding RNA IPW regulates the imprinted DLK1-DIO3 locus in an induced pluripotent stem cell model of Prader-Willi syndrome

Supplementary Figure 1

(a) G-band karyotyping of representative PWS-iPSC lines, demonstrating a diploid genome, excluding chromosome 15 aberrations. (b) Representative alkaline phosphatase staining for PWS-iPSC lines. Scale bar, 200 μm. (c) Mean expression levels ± s.d. of representative pluripotency markers in four PWS-iPSC lines (PWS-iPSC-1-A and B and PWS-iPSC-2-A and B) compared with five control (WT) PSCs (two iPSC and three ESC lines). (d) Gene expression analysis of 20-d-old embryoid bodies (EBs) from the two PWS-iPSC lines, demonstrating differentiation in vitro into all three embryonic germ layers. DES, NCAM1 and AFP represent markers for mesoderm, ectoderm and endoderm, respectively (mean expression levels ± s.d.; two biological replicates). (e) Mean expression levels ± s.d. of representative potential targets of PEGs in the PWS locus, in PWS and control (WT) fibroblasts.