Supplementary Figure 6 : Chromatin regulation in PWS-iPSCs.

From: The noncoding RNA IPW regulates the imprinted DLK1-DIO3 locus in an induced pluripotent stem cell model of Prader-Willi syndrome

Supplementary Figure 6

(a) qRT-PCR of the mean relative fold change ± s.d. (three biological replicates each) in HOTAIR in PWS-iPSCs before and after transfection with an overexpression plasmid. (b) qRT-PCR of the mean relative fold change ± s.d. (three biological replicates each) in MEG3 in PWS-iPSCs before and after transfection with the HOTAIR overexpression plasmid, relative to control (WT) PSCs. (c) Allelic sequence analysis following ChIP for H3K9me3 reveals the association of this histone modification with both methylated and unmethylated alleles of the IG-DMR in normal PSCs. (d) qRT-PCR of the mean relative fold change ± s.d. in ZFP57 in Pg-iPSCs (three biological replicates each) and PWS-iPSCs (three biological replicates each) following introduction of IPW, relative to control (WT) iPSCs.