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XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells

Nature Genetics volume 45, pages 239241 (2013) | Download Citation

Abstract

X-chromosome inactivation (XCI) in mammals relies on XIST, a long noncoding transcript that coats and silences the X chromosome in cis. Here we report the discovery of a long noncoding RNA, XACT, that is expressed from and coats the active X chromosome specifically in human pluripotent cells. In the absence of XIST, XACT is expressed from both X chromosomes in humans but not in mice, suggesting a unique role for XACT in the control of human XCI initiation.

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Acknowledgements

We thank members of our laboratory and M. Lalande for stimulating discussion and critical reading of the manuscript. The research leading to these results has received funding from the European Research Council (ERC) under the European Community's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement 206875 and the INSERM (Avenir Program R0721HS).

Author information

Affiliations

  1. Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate, Paris, France.

    • Céline Vallot
    • , Christophe Huret
    •  & Claire Rougeulle
  2. Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 7216 Epigenetics and Cell Fate, Paris, France.

    • Céline Vallot
    • , Christophe Huret
    •  & Claire Rougeulle
  3. Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université de Lyon, Université Lyon 1, Villeurbanne, France.

    • Yann Lesecque
    •  & Laurent Duret
  4. Stem Cell Institute, University of Connecticut Health Center, Farmington, Connecticut, USA.

    • Alissa Resch
  5. ESTeam Paris Sud, Institut National de la Santé et de la Recherche Médicale (INSERM) U935, Université Paris Sud 11, Assistance Publique–Hôpitaux de Paris (AP-HP), Villejuif, France.

    • Noufissa Oudrhiri
    •  & Annelise Bennaceur-Griscelli

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Contributions

C.V. and C.H. performed the experiments. Y.L. and L.D. did the bioinformatic analysis of XACT conservation. N.O. and A.B.-G. provided the H9-MSC-iPS system. A.R. contributed to the bioinformatics analysis of the RNA-seq data. C.V. and C.R. conceived and designed the experiments, and wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Claire Rougeulle.

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DOI

https://doi.org/10.1038/ng.2530

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