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Genetic control of rice plant architecture under domestication

Abstract

The closely related wild rice species Oryza rufipogon is considered the progenitor of cultivated rice (Oryza sativa)1,2,3,4,5. The transition from the characteristic plant architecture of wild rice to that of cultivated rice was one of the most important events in rice domestication; however, the molecular basis of this key domestication transition has not been elucidated. Here we show that the PROG1 gene controls aspects of wild-rice plant architecture, including tiller angle and number of tillers. The gene encodes a newly identified zinc-finger nuclear transcription factor with transcriptional activity and is mapped on chromosome 7. PROG1 is predominantly expressed in the axillary meristems, the site of tiller bud formation. Rice transformation experiments demonstrate that artificial selection of an amino acid substitution in the PROG1 protein during domestication led to the transition from the plant architecture of wild rice to that of domesticated rice.

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Figure 1: Plant architecture phenotypic characterization in Teqing and NIL(PROG1).
Figure 2: Map-based cloning of PROG1 and genetic complementation test.
Figure 3: PROG1 structure and mutation analysis.
Figure 4: Subcellular localization, transcription activity assay and expression pattern analysis of PROG1.

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Acknowledgements

We thank Z.-Z. Piao and X.-M. Li for technical assistance. We thank S. Luan for critically reading the manuscript. This work was supported by grants from the Ministry of Science and Technology of China, the National Natural Science Foundation of China, the Chinese Academy of Sciences and the Shanghai Science and Technology Development Fund to H.-X.L.

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Authors and Affiliations

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Contributions

H.-X.L. designed the experiments. J.J. performed most of the experiments. W.H., J.-P.G., J.Y., M.S., M.-Z.Z., D.L. and H.-X.L. performed some of the experiments. H.-X.L. wrote the manuscript.

Corresponding author

Correspondence to Hong-Xuan Lin.

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Supplementary Figures 1–3, Supplementary Tables 1 and 2 (PDF 149 kb)

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Jin, J., Huang, W., Gao, JP. et al. Genetic control of rice plant architecture under domestication. Nat Genet 40, 1365–1369 (2008). https://doi.org/10.1038/ng.247

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  • DOI: https://doi.org/10.1038/ng.247

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