Shigella are human-adapted Escherichia coli that have gained the ability to invade the human gut mucosa and cause dysentery1,2, spreading efficiently via low-dose fecal-oral transmission3,4. Historically, S. sonnei has been predominantly responsible for dysentery in developed countries but is now emerging as a problem in the developing world, seeming to replace the more diverse Shigella flexneri in areas undergoing economic development and improvements in water quality4,5,6. Classical approaches have shown that S. sonnei is genetically conserved and clonal7. We report here whole-genome sequencing of 132 globally distributed isolates. Our phylogenetic analysis shows that the current S. sonnei population descends from a common ancestor that existed less than 500 years ago and that diversified into several distinct lineages with unique characteristics. Our analysis suggests that the majority of this diversification occurred in Europe and was followed by more recent establishment of local pathogen populations on other continents, predominantly due to the pandemic spread of a single, rapidly evolving, multidrug-resistant lineage.
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We thank M. Levine (University of Maryland School of Medicine) and C. Tang (University of Oxford) for their kind gift of S. sonnei strain 53G. This work was supported by the Wellcome Trust (0689) and a Victorian Life Sciences Computation Initiative (VLSCI) grant (VR0082) on its Peak Computing Facility at the University of Melbourne (an initiative of the Victorian Government, Australia). K.E.H. was supported by a Fellowship from the National Health & Medical Research Council (NHMRC) of Australia (628930); S.B. is supported by an Oak Foundation Fellowship through Oxford University (OAKF9) and by the Li Ka Shing foundation (LG13); F.X.W. was partially funded by the Institut de Veille Sanitaire; J.Y. was supported by a UK Medical Research Council (MRC) grant (G0800173); and D.W.K. was partially supported by grant RTI05-01-01 from the Korean Ministry of Knowledge and Economy (MKE).
The authors declare no competing financial interests.
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Holt, K., Baker, S., Weill, FX. et al. Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemination from Europe. Nat Genet 44, 1056–1059 (2012). https://doi.org/10.1038/ng.2369
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