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Variant between CPT1B and CHKB associated with susceptibility to narcolepsy

Abstract

Narcolepsy (hypocretin deficiency), a sleep disorder characterized by sleepiness, cataplexy and rapid eye movement (REM) sleep abnormalities, is tightly associated with HLA-DRB1*1501 (M17378) and HLA-DQB1*0602 (M20432). Susceptibility genes other than those in the HLA region are also likely involved. We conducted a genome-wide association study using 500K SNP microarrays in 222 Japanese individuals with narcolepsy and 389 Japanese controls, with replication of top hits in 159 Japanese individuals with narcolepsy and 190 Japanese controls, followed by the testing of 424 Koreans, 785 individuals of European descent and 184 African Americans. rs5770917, a SNP located between CPT1B and CHKB, was associated with narcolepsy in Japanese (rs5770917[C], odds ratio (OR) = 1.79, combined P = 4.4 × 10−7) and other ancestry groups (OR = 1.40, P = 0.02). Real-time quantitative PCR assays in white blood cells indicated decreased CPT1B and CHKB expression in subjects with the C allele, suggesting that a genetic variant regulating CPT1B or CHKB expression is associated with narcolepsy. Either of these genes is a plausible candidate, as CPT1B regulates β-oxidation, a pathway involved in regulating theta frequency during REM sleep, and CHKB is an enzyme involved in the metabolism of choline, a precursor of the REM- and wake-regulating neurotransmitter acetylcholine.

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Figure 1: Genome-wide association results for 222 Japanese narcoleptic individuals and 389 Japanese controls.
Figure 2: Pairwise linkage disequilibrium (D′) diagram for CPT1B and CHKB.
Figure 3: Expression analysis of CPT1B and CHKB.

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Acknowledgements

We would like to express our sincere thanks to the study participants. This study was supported by Grants-in-Aid for Scientific Research on Priority Areas “Comprehensive Genomics” and “Applied Genomics” from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Grant-in-Aid for JSPS fellows, and US National Institutes of Health grant NS 23724 to E. Mignot, a Howard Hughes Investigator.

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Authors and Affiliations

Authors

Contributions

Study design: T.M., M.K., E.M. and K.T.; sample collection: T.M., M.K., L.L., S.-C.H., J.F., Y.-K.S., J.-H.J., Y.O., S.T., M.H., Y.H. and E.M.; genotyping: T.M., M.K., N.N., M.S., L.L., J.F., E.M. and K.T.; statistical analysis: T.M., J.O., R.K., A.F., S.M., T.S., M.H., E.M. and K.T.; real-time quantitative RT-PCR: T.M. and M.H.; manuscript writing: T.M., M.K., E.M. and K.T.

Corresponding author

Correspondence to Katsushi Tokunaga.

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Supplementary Tables 1–4, Supplementary Figures 1 and 2 (PDF 67 kb)

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Miyagawa, T., Kawashima, M., Nishida, N. et al. Variant between CPT1B and CHKB associated with susceptibility to narcolepsy. Nat Genet 40, 1324–1328 (2008). https://doi.org/10.1038/ng.231

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