Presence of Glutamate Synthase in Fission Yeasts and its Possible Role in Ammonia Assimilation

Nature New Biology volume 246, pages115–116(1973)Cite this article

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Abstract

IT is generally agreed that glutamate is a key intermediate in the assimilation of ammonia by yeasts. This has been demonstrated particularly in Candida utilis by Sims and Folkes1 and in Saccharornyces cerevisiae by Jones, Pragnell and Pierce2. These workers followed the incorporation of ammonia into cell material using 15N as label, and established that only glutamate and glutamine derived their α-amino nitrogen directly from ammonia, and were synthesised at a rate sufficient to supply the α-amino nitrogen required for cellular growth. All other amino acids were synthesised by way of transamination reactions.

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References

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Author notes

    • VALERIE J. BURN

    Present address: Department of Biological Sciences, University of Dundee, DD1 4HN

Affiliations

  1. Department of Microbiology, University of Newcastle upon Tyne, NE1 7RU

    • C. M. BROWN
    • , VALERIE J. BURN
    •  & B. JOHNSON

  2. Department of Biological Sciences, University of Dundee, DD1 4HN

    • VALERIE J. BURN

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BROWN, C., BURN, V. & JOHNSON, B. Presence of Glutamate Synthase in Fission Yeasts and its Possible Role in Ammonia Assimilation. Nature New Biology 246, 115–116 (1973) doi:10.1038/newbio246115a0

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