Abstract
RECENT studies in microorganisms indicate the presence of RNA in nascent DNA and point to a role of the RNA as primer for DNA synthesis1–8. We report here evidence that RNA is also present in human cells in short chain intermediates of DNA replication, apparently serving a primer function as in microorganisms.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Brutlag, D., Schekman, R., and Komberg, A., Proc. nat. Acad. Sci. U.S.A., 68, 2826 (1971).
Wickner, W., Brutlag, D., Schekman, R., and Kornberg, A., Proc. natn. Acad. Sci. U.S.A., 69, 965 (1972).
Schekman, R., Wickner, W., Westergaard, O., Brutlag, D., Geider, K., Bertsch, L. L., and Kornberg, A., Proc. natn. Acad. Sci. U.S.A., 69, 2691 (1972).
Sugino, A., Hirose, S., and Okazaki, R., Proc. natn. Acad. Sci. U.S.A., 69, 1863 (1972).
Sugino, A., and Okazaki, R., Proc. natn. Acad. Sci. U.S.A., 70, 88 (1973).
Blair, D. G., Sherratt, D. J., Clewell, D. B., and Helinski, D. R., Proc. natn. Acad. Sci. U.S.A., 49, 2518 (1972).
Magnusson, G., Pigiet, V., Winnacker, E. L., Abrams, R., and Reichard, P., Proc. natn. Acad. Sci. U.S.A., 70, 412 (1973).
Waqar, M. A., and Huberman, J. A., Biochem. biophys. Res. Commun., 51, 174 (1973).
Painter, R. B., and Schaefer, A., Nature, 221, 1215 (1969).
Habener, J. F., Bynum, B. S., and Shack, J., J. molec. Biol., 49, 157 (1970).
Schandl, E. K., and Taylor, J. H., Biochem. biophys. Res. Commun., 34, 291 (1969).
Sato, S., Tanaka, M., and Sugimura, T., Biochim. biophys. Acta, 209, 43 (1970).
Berger, H., jun., and Irvin, J. L., Proc. natn. Acad. Sci. U.S.A., 65, 152 (1970).
Bernardi, G., Nature, 206, 779 (1965).
Nuzzo, F., Brega, A., and Falaschi, A., Proc. natn. Acad. Sci. U.S.A., 65, 1017 (1970).
Hyodo, M., Koyama, H., and Ono, T., Expl. Cell Res., 67, 461 (1971).
Schandl, E. K., and Taylor, J. H., Biochim. biophys. Acta, 228, 595 (1971).
Kidwell, W. R., and Mueller, G. C., Biochem. biophys. Res. Commun., 36, 756 (1969).
Probst, H., Ullrich, A., and Krauss, G., Biochim. biophys. Acta, 254, 15 (1971).
Chiu, S. F., and Rauth, A. M., Biochim. biophys. Acta, 259, 164 (1972).
Sato, S., Ariake, S., Saito, M., ., and Sugimura, T., Biochem. biophys. Res. Commun., 49, 270 (1972).
Okazaki, R., Okazaki, T., Sakabe, K., Sugimoto, K., Kainuma, R., Sugino, A., and Iwatsuki, N., Cold Spring Harb. Symp. quant. Biol., 28, 129 (1968).
Sato, S., Ariake, S., Saito, M., and Sugimura, T., Biochem. biophys. Res. Commun., 49, 827 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FOX, R., MENDELSOHN, J., BARBOSA, E. et al. RNA in Nascent DNA from Cultured Human Lymphocytes. Nature New Biology 245, 234–237 (1973). https://doi.org/10.1038/newbio245234a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio245234a0
This article is cited by
-
Particular RNA primer from growth medium differentially stimulates in vitro DNA synthesis and in vivo cell growth of Neurospora crassa and its slime mutant
Current Genetics (1987)
-
Continuous synthesis of long DNA chains by chick embryo DNA polymerase γ
Nature (1980)
-
Ribonucleotides in DNA newly synthesised in 3T6 cells in vivo
Nature (1979)
-
In vitro DNA synthesis in a concentrated yeast lysate
Molecular and General Genetics MGG (1979)
-
RNA linked membrane associated DNA
Molecular and Cellular Biochemistry (1974)
