Abstract
RAPIDLY labelled, high molecular weight RNA, with a DNA-like base composition, is synthesized on oocyte lampbrush chromosomes of the newt, Triturus cristatus carnifex1. This RNA resembles the heterogeneous nuclear RNA (HnRNA) found in other systems2, and when isolated is found to be associated with large amounts (>97%) of non-basic proteins in the form of ribonucleoprotein (RNP) particles which are freed from the chromosomes1. Isolation of the particles at high ionic strength (0.5 M NaCl) does not significantly reduce their protein content1, indicating that the protein constituents of the particles are not simply adventitiously bound during isolation3. The relationship between newly synthesized RNA and specific proteins may well be important as a means of control of RNA processing4,5 and potential translation in the cytoplasm. Such mechanisms are of particular interest in developmental systems such as newt oogenesis, where there appears to be considerable storage of informational RNA6.
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SOMMERVILLE, J., HILL, R. Proteins Associated with Heterogeneous Nuclear RNA of Newt Oocytes. Nature New Biology 245, 104–106 (1973). https://doi.org/10.1038/newbio245104a0
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DOI: https://doi.org/10.1038/newbio245104a0
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