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Isolation and Genetic Localization of Three ϕX174 Promoter Regions

Nature New Biology volume 243, pages 233–236 (1973)Cite this article

An Addendum to this article was published on 20 June 1973

Abstract

SPECIFIC sequences of nucleic acids such as ribosome binding sites1 (W. Gilbert, cited in ref. 2) and portions of the promoter region3,4 have been isolated by protecting those sequences with the relevant protein or organelle and digesting away the exposed nucleic acid. We have isolated specific sequences from the promoter regions in bacteriophage ϕX174 replicative form (RF) DNA using RNA polymerase as the protecting protein. We wish to describe that isolation and the procedures used to localize these RNA-polymerase-protected sequences within the ϕX174 genome.

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Authors and Affiliations

  1. Department of Bacteriology and Immunology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, 27514

    CHENG-YIEN CHEN, CLYDE A. HUTCHISON III & MARSHALL HALL EDGEL

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  1. CHENG-YIEN CHEN
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  2. CLYDE A. HUTCHISON III
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  3. MARSHALL HALL EDGEL
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CHEN, CY., HUTCHISON, C. & EDGEL, M. Isolation and Genetic Localization of Three ϕX174 Promoter Regions. Nature New Biology 243, 233–236 (1973). https://doi.org/10.1038/newbio243233a0

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  • DOI: https://doi.org/10.1038/newbio243233a0

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