Abstract
GENTAMICIN is an important antibiotic with potentially serious toxicity. The range between therapeutic effectiveness and toxicity is narrow and the dosage must be carefully monitored. Ototoxicity and nephrotoxicity are more common if the peak serum levels are greater than 12 µg/ml.1, while peak serum levels below 4 µg/ml. are, in some instances2, inadequate for bacterial control. Existing methods for the measurement of this, and other antibiotics, rely on microbiological assays which determine antibacterial activity in dilutions of the test fluid. These assays lack sensitivity, specificity and precision and have not been extensively used in clinical medicine. Radioimmunoassays have provided practical and widely applied methods for the measurement of hormones3 and digitalis glycosides4. These assays possess the technical simplicity, sensitivity and specificity lacking in microbiological assays. This report describes the development of a radioimmunoassay for gentamicin, a potentially toxic antibiotic.
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LEWIS, J., NELSON, J. & ELDER, H. Radioimmunoassay of an Antibiotic: Gentamicin. Nature New Biology 239, 214–216 (1972). https://doi.org/10.1038/newbio239214a0
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DOI: https://doi.org/10.1038/newbio239214a0
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