Abstract
THE molecular mechanism for initiating DNA replication can be studied using a subcellular system. Rao and Johnson1 found that HeLa cells in the pre-DNA-synthetic (G-1) period of the cell cycle initiate DNA synthesis after fusion with cells that are in the DNA synthetic (S) period. A previous subcellular system of DNA replication from HeLa cells2–4 consisted of intact nuclei, supplemented with the four deoxy-nucleoside triphosphates, salt, ATP and a cytosol factor. The nuclei in this system appeared to be permeable to proteins and DNA synthesis was very similar to that within intact cells. We report here the initiation of DNA synthesis in nuclei isolated from HeLa cells. Our results suggest that, with the synchronization method used, a small percentage of dormant G-1 nuclei can be stimulated by S-phase cytoplasm; this would be the case if the cells were receptive to stimulation for only 30–60 min during the cell cycle.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Rao, P. N., & Johnson, R. T., Nature, 225, 159 (1970).
Friedman, D. L., & Mueller, G. C., Fed. Proc., 26, 868 (1967).
Friedman, D. L., & Mueller, G. C., Biochim. Biophys. Acta, 161, 455 (1968).
Kidwell, W. R., & Mueller, G. C., Biochem. Biophys. Res. Comm., 36, 756 (1969).
Thompson, L. K., & McCarthy, B. J., Fed. Proc., 30, 1177 (1971).
Painter, R. B., Cur. Topics Rad. Res., 7, 45 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KUMAR, K., FRIEDMAN, D. Initiation of DNA Synthesis in HeLa Cell-free System. Nature New Biology 239, 74–76 (1972). https://doi.org/10.1038/newbio239074a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio239074a0