Abstract
THE unusual modified base1 of the nucleic acid of the large, generalized transducing phage of Bacillus subtilis SP-15 was predicted from the low denaturation temperature of the native nucleic acid. The base was detected as a fifth ultraviolet-absorbing component once the nucleic acid had been hydrolysed with formic acid; it partially replaces the thymine and represents 12 mol per cent of all the bases1. It is responsible for the low Tm of the nucleic acid, since once all other unusual components have been removed, the remaining material melts at the same temperature as the native nucleic acid. We report here the isolation and characterization of this new pyrimidine.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Marmur, J., Brandon, C., Neubort, S., Ehrlich, M., Mandel, M., & Konvicka, J. J., Nature New Biology, 239, 68 (1972).
Wyatt, G. R., Biochem. J., 48, 584 (1951).
Marshall, J. R., & Walker, J., J. Chem. Soc., 1004 (1951).
Budzikiewicz, H., Djerassi, C., & Williams, D. H., Mass Spectrometry of Organic Compounds, 84 (Holden-Day, 1967).
Porter, Q. N., & Baldas, J., Mass Spectrometry of Heterocyclic Compounds (edit. by Weissberger, A., and Taylor, E. C), 474 (Wiley-Interscience, New York, 1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BRANDON, C., GALLOP, P., MARMUR, J. et al. Structure of a New Pyrimidine from Bacillus subtilis Phage SP-15 Nucleic Acid. Nature New Biology 239, 70–71 (1972). https://doi.org/10.1038/newbio239070a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/newbio239070a0
This article is cited by
-
An atypical deoxynucleoside triphosphate inBacillus subtilis infected with bacteriophage SP10c
Current Microbiology (1979)
-
Unique Properties of Nucleic Acid from Bacillus subtilis Phage SP-15
Nature New Biology (1972)