Abstract
The following two articles describe successful syntheses in vitro of DNA copies of various mammalian globin mRNAs, using reverse transcriptase from avian myoblastosis virus. Short stretches of poly(dT) greatly enhance this effect, presumably because they prime correct initiation of reverse transcription of these mRNAs by pairing with known poly (A) sequences at their 3′-ends.
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VERMA, I., TEMPLE, G., FAN, H. et al. In vitro Synthesis of DNA Complementary to Rabbit Reticulocyte 10S RNA. Nature New Biology 235, 163–167 (1972). https://doi.org/10.1038/newbio235163a0
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DOI: https://doi.org/10.1038/newbio235163a0
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