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Natural Read-through at the UGA Termination Signal of Qβ Coat Protein Cistron

Nature New Biology volume 234, pages 206–209 (1971)Cite this article

Abstract

GENETIC analysis of conditional lethal mutants of Qβ, a small RNA-containing bacteriophage of Escherichia coli, has demonstrated the existence of three cistrons1. These have been correlated2 with three of the four phage-specific proteins observed after Qβ infection2–4: gene 1 codes for the phage-specific subunit of Qβ replicase, gene 2 for the maturation protein, and gene 3 for the major coat protein. Using SDS-polyacrylamide gel electrophoresis to characterize these polypeptides, Garwes et al. introduced the nomenclature I, IIa and III for the phage-specific proteins3; the corresponding molecular weights are 64,000, 41,000, and 14,000.

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  1. Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138

    ALAN M. WEINER & KLAUS WEBER

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WEINER, A., WEBER, K. Natural Read-through at the UGA Termination Signal of Qβ Coat Protein Cistron. Nature New Biology 234, 206–209 (1971). https://doi.org/10.1038/newbio234206a0

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  • DOI: https://doi.org/10.1038/newbio234206a0

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