Abstract
MESSENGER-SPECIFIC initiation factors, capable of discriminating between classes of messenger RNAs (mRNAs) or different cistrons in viral RNA, have been implicated in the regulation of protein synthesis in bacteria1–5. Comparable but less detailed observations have also been made in eukaryotic systems6–10. For example, RNA extracted from a mammalian virus (encephalomyocarditis virus, EMC) cannot be translated in a reticulocyte cell-free system unless the system is fortified with an extract from responsive cells—in this case, Krebs II ascites cells6. Such results imply the existence of tissue-specific factors, and lead to questions whether this incompatibility is reciprocated by an inability of the Krebs II ascites cell system to respond to the mRNA for globin.
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MATHEWS, M., OSBORN, M. & LINGREL, J. Translation of Globin Messenger RNA in a Heterologous Cell-free System. Nature New Biology 233, 206–209 (1971). https://doi.org/10.1038/newbio233206a0
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DOI: https://doi.org/10.1038/newbio233206a0
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