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Identification of Mouse Haemoglobin Messenger RNA

Nature New Biology volume 233, pages 204–206 (1971)Cite this article

Abstract

RETICULOCYTE polyribosomes contain 9S RNA with many of the properties expected for the haemoglobin messenger RNA (mRNA)1–12. Proof that this RNA is the haemoglobin (Hb) mRNA, however, can be obtained only by showing that it directs the synthesis of globin chains. Laycock and Hunt13 added an RNA isolated from rabbit reticulocytes to an E. coli cell-free preparation and observed the synthesis of material, with the properties of globin in the presence of N-acetylvalyl tRNA. We added the mouse reticulocyte 9S RNA to a rabbit reticulocyte cell-free system and have shown that material is synthesized which co-chromatographs with mouse globin β-chains14. We now present evidence that the material synthesized under the direction of the mouse 9S RNA is indeed mouse haemoglobin β-chains.

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Authors and Affiliations

  1. Department of Biological Chemistry, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45219

    RAYMOND E. LOCKARD & JERRY B. LINGREL

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  1. RAYMOND E. LOCKARD
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  2. JERRY B. LINGREL
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LOCKARD, R., LINGREL, J. Identification of Mouse Haemoglobin Messenger RNA. Nature New Biology 233, 204–206 (1971). https://doi.org/10.1038/newbio233204a0

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