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Isolation of Catenated Forms of R Factor DNA from Minicells

Nature New Biology volume 231, pages 249–252 (1971)Cite this article

Abstract

STUDIES1–6 of the molecular nature of antibiotic resistance (R) factors in Escherichia coli have shown that several of them consist of covalently closed molecules of circular DNA. Use was made of this property in the separation of the R factor from chromosomal DNA of E. coli, which has a similar nucleotide-base composition. In selecting for covalently circular R factor DNA molecules, however, the procedures used in these earlier experiments-caesium chloride-ethidium bromide centrifuga-tion7 and bulk nitrocellulose adsorption2—necessarily selected against isolation of other (non-circular) forms of R factor DNA that might have been present.

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Authors and Affiliations

  1. Department of Medicine, Stanford University School of Medicine, Stanford, California, 94305

    STANLEY N. COHEN, RICHARD P. SILVER & ANNETTE E. MCCOUBREY

  2. Gates, Crellin and Church Laboratories of Chemistry, California Institute of Technology, Pasadena, California, 91109

    PHILLIP A. SHARP

Authors
  1. STANLEY N. COHEN
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  2. RICHARD P. SILVER
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  3. ANNETTE E. MCCOUBREY
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  4. PHILLIP A. SHARP
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COHEN, S., SILVER, R., MCCOUBREY, A. et al. Isolation of Catenated Forms of R Factor DNA from Minicells. Nature New Biology 231, 249–252 (1971). https://doi.org/10.1038/newbio231249a0

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