Sensitive and Rapid Assay for L-Asparaginase

Article metrics

Abstract

L-ASPARAGINASE (L-asparagine amidohydrolase EC 3.5.1.1) from various sources has anti-tumorigenic properties1. Its presence confers on guinea-pig serum activity against experimental acute lymphatic leukaemia2. Most available asparaginase preparations are derived from microorganisms. In particular, two enzymes of Escherichia coli have been studied and one of them (EC2) has induced remissions in numerous patients with acute lymphatic leukaemia3. After relapse, however, different treatment is often necessary. This suggests that various microorganisms should be screened for effective producers of L-asparaginase, immunologically different from that of E. coli. A simple and sensitive assay method would assist such a screening and the subsequent purification of the enzyme.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Adamson, R. H., and Fabro, S., Cancer Chemotherapy Rep. (Part I), 52, 617 (1968).

  2. 2

    Broome, J. O., Nature, 191, 1114 (1961).

  3. 3

    Oettgen, H. F., Old, L. J., Boyse, E. A., Campbell, H. A., Philips, F. S., Clarkson, B. D., Tallal, L., Leeper, R. D., Schwartz, M. K., and Kim, J. H., Cancer Research, 27, 2619 (1967).

  4. 4

    Grossowicz, N., Wainfan, E., Borek, E., and Waelsch, H., J. Biol. Chem., 187, 111 (1950).

  5. 5

    Lipmann, F., and Tuttle, L. C., J. Biol. Chem., 159, 21 (1945).

  6. 6

    Schwartz, J. H., Cedar, M., and Ehrman, M., Fed. Proc., 29, 407 Abs. (1970).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

FROHWEIN, Y., FRIEDMAN, M., REIZER, J. et al. Sensitive and Rapid Assay for L-Asparaginase. Nature New Biology 230, 158–159 (1971) doi:10.1038/newbio230158a0

Download citation

Further reading