Figure 5: STOML3 modulates Piezo1 and Piezo2 channels activity via association with cholesterol. | Nature Communications

Figure 5: STOML3 modulates Piezo1 and Piezo2 channels activity via association with cholesterol.

From: Membrane stiffening by STOML3 facilitates mechanosensation in sensory neurons

Figure 5

(a,c,e) Representative traces of mechanically gated currents in N2a cells (a) or N2a cells expressing STOML3 (c) or N2a cells expressing STOML3-P40S mutant (e) treated with or without MβCD at the given indentation (4.2 μm). (b) When N2a cells were treated with MβCD (n=33), mechanosensitivity was significantly reduced at 4.2 μm displacement compared with cells without MβCD treatment (n=27) (two-way ANOVA with post hoc Bonferroni’s test, P>0.05). (d) MβCD significantly reduced the mechanosensitive currents in N2a cells overexpressing STOML3 (STOML3: n=11, STOML3+MβCD: n=8; two-way ANOVA with post hoc Bonferroni’s test, P<0.0001). (f) MβCD did not alter the mechanosensitive currents in N2a cells expressing STOML3-P40S mutant (STOML3-P40: n=6, STOML3-P40+MβCD: n=7; two-way ANOVA with post hoc Bonferroni’s test, P>0.05). (g) Representative traces of mechanically gated currents in HEK293 cells expressing Piezo2 and STOML3 treated with or without MβCD at the given indentation (4.2 μm). (h) MβCD treatment significantly reduced the potentiation effect of STOML3 on Piezo2-mediated mechanosensitive currents in HEK293 cells, while STOML3-P40S mutant failed to potentiate Piezo2 currents (two-way ANOVA with post hoc Bonferroni’s test; STOML3 effect: Piezo2 versus Piezo2+STOML3, P<0.0001, black *; MβCD effect: Piezo2+STOML3 versus Piezo2+STOML3+MβCD, P<0.0001, pink *; STOML3-P40S effect: Piezo2 versus Piezo2+STOML3-P40S, P>0.05; Piezo2: n=9, Piezo2+STOML3: n=7, Piezo2+STOML3+MβCD: n=5, Piezo2+STOML3-P40S: n=7). **P<0.01, ***P<0.001, ****P<0.0001. Error bars indicate s.e.m.

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