Dendrograms relating relative development of mutations from inferred common ancestors (dashed, white rounded boxes) and tumour zones (solid boxes) for each (a) AIS and (b) MIA tumour. A complete list of mutations in each patient is provided in the white ovals. Mutations in common ancestors are shown in black, while subsequent alterations are depicted in red for mutations and blue for copy number variations. Zones with no mutation (zones 1 and 2 for AIS patient #2 and zone 2 for AIS patient #4) are shown as parallel branches, but may also be ancestors of subsequent mutations in other zones. Synonymous and non-coding mutations were not considered in the analyses.