Figure 4 : Effect of DSS-induced GI tract damage on systemic inflammation and immune activation within the distal AxLN.

From: Experimental colitis in SIV-uninfected rhesus macaques recapitulates important features of pathogenic SIV infection

Figure 4

Representative images of (a) Mx1- and (b) P-STAT1-stained AxLNs showing limited inflammation in untreated control RMs and increased inflammation in DSS-treated RMs. Scale bars = 100 μm. Quantification of the percent area of the AxLN positive for (c) Mx1 and (d) P-STAT1. Direct positive correlation between the extent of GI tract epithelial damage and the magnitude of (e) Mx1 and (f) P-STAT1 expression in AxLNs. Lines are based on linear regression and r and P values are based on Spearman rank correlation coefficient. Longitudinal analysis of (g) CD4+Ki67+ and (h) CD8+Ki67+ in the peripheral blood mononuclear cells (PBMC) pre- and post-DSS treatment. P values are based on the Mann–Whitney test (c,d) or the Wilcoxon matched pairs test (g,h). Untreated control RMs (blue circles) n=4 (eh) to 6 (c,d) and acute DSS-treated RMs (red circles) n=6, with individual shapes representing individual animals (g,h). Bar graphs show group means±s.e.m. with individual animal data points shown.