Memory reconsolidation is considered to be the process whereby stored memories become labile on recall, allowing updating. Blocking the restabilization of a memory during reconsolidation is held to result in a permanent amnesia. The targeted knockdown of either Zif268 or Arc levels in the brain, and inhibition of protein synthesis, after a brief recall results in a non-recoverable retrograde amnesia, known as reconsolidation blockade. These experimental manipulations are seen as key proof for the existence of reconsolidation. However, here we demonstrate that despite disrupting the molecular correlates of reconsolidation in the hippocampus, rodents are still able to recover contextual memories. Our results challenge the view that reconsolidation is a separate memory process and instead suggest that the molecular events activated initially at recall act to constrain premature extinction.
Reconsolidation theory proposes that stable, consolidated memories under certain experimental conditions can enter into a labile state on recall, allowing the updating or strengthening of the stored memory1,2. These memories must then be actively restabilized through a process of reconsolidation otherwise they are lost, resulting in a permanent amnesia3,4. Memory recall is accompanied by specific molecular processes, including the expression of the genes Zif268 and Arc and the synthesis of new proteins. Disruption of these molecular processes following memory recall in conditioning studies has been shown to result in a permanent amnesia, and this is considered to be the key experimental demonstration of the existence of reconsolidation as a separate memory process4,5.
On the other hand, memory recall also has the potential to initiate extinction of the memory6. Extinction is considered to be a new learning process in which stimuli gain new predictive properties. As such, extinction memories come to compete for behavioural control at recall and the behaviour driven by the original memory is suppressed7,8. Notably, following extinction, memory-driven behaviours can later be revealed by experimental manipulations such as a reminder stimulus9. In conditioning studies, extinction is engaged when a conditioned association is repeatedly retrieved or recalled for an extended period without further reinforcement10,11. During recall, a careful balance must be achieved in maintaining the original memory and initiating extinction. It is therefore possible that the molecular processes accompanying the initial phase of memory recall act to constrain the premature engagement of extinction rather than to mediate reconsolidation.
In our contextual fear conditioning protocol in rats, targeted knockdown of plasticity and memory-associated molecules in the dorsal hippocampus during a brief recall trial resulted in the loss of expression of fear memory5. We sought to disambiguate between two alternative explanations of the role of the molecular events accompanying early memory recall (mediating reconsolidation versus constraining extinction) by the use of a reminder stimulus. The reminder stimulus was a low intensity unconditioned stimulus (US), below the intensity required to produce de novo conditioning (Supplementary Fig. 1)12,13. The expression of a fear memory after extinction should be reinstated by such a reminder US9; however, an experimental manipulation that disrupts reconsolidation should result in a permanent amnesia4, and as a consequence, the memory should not be recoverable by a reminder.
Here, we show that memory recall targeted knockdown of protein synthesis and the expression of reconsolidation-associated molecules Zif268 and Arc with directly infused antisense oligodeoxynucleotide (ASO) agents that are used routinely to define reconsolidation blockade, do not result in a permanent amnesia because fear memory can be recovered after a reminder US. These results call us to reassess the phenomena of memory reconsolidation as a constructive, independent mnemonic process and instead suggest that an active process at recall in the dorsal hippocampus acts to constrain the extinction of memories, rather than to facilitate reconsolidation.
The transcription factor Zif268 is rapidly expressed following memory recall14 and has been argued to be a key molecular correlate of reconsolidation5. We have previously shown that hippocampal infusion of ASO to Zif268 during a 2-min short recall (SR) session results in the loss of a recently acquired contextual fear memory (CFM). Notably, the Zif268-specific loss of CFM was robust and persistent and did not undergo spontaneous or state-dependent recovery suggesting a permanent amnesia consistent with a role of Zif268 in reconsolidation5. However, we now show that CFM can be recovered after ZIFASO infusion by interposing a reminder stimulus at a subsequent recall trial (Fig. 1). The recovery of the conditioned response (CR) by the reminder was not due to the strengthening of the residual memory, a Zif268-dependent process15, because reinstatement was also seen when ZIFASO was administered before the reminder session. Also, reinstatement of the CR by the reminder cue occurred when ZIFASO alone or when combined infusions of ZIFASO and BDNFASO were administered before the reminder US (Supplementary Fig. 2a,b), demonstrating that the recovery of the CR was not due to additional incremental learning by the reminder US16, because BDNFASO blocks new learning in the absence5 and presence of ZIFASO (Supplementary Fig. 2c). In addition, the ASO infusions are unlikely to be causing non-specific effects in the hippocampus because retrieval of CFM is intact. Later, the rats could discriminate between the conditioned context and a new context, and could support this conditioning to a new context (Fig. 1a,b) and there is also no evidence of gross damage to the dorsal hippocampus after histological examination17. Thus, the experimental manipulation is consistent with the ZIFASO targeting extinction, therefore, implicating Zif268 to act on constraining premature extinction, opposed to mediating reconsolidation.
Arc (Activity-regulated cytoskeletal-associated protein) has also been argued to play an essential role in fear memory reconsolidation in the amygdala18. Here we confirm that Arc expression is rapidly increased in the hippocampal CA1 region after the recall of CFM, in agreement with other studies19,20,21 (Fig. 2a–c). Furthermore, the increase in Arc expression was greater following the SR session compared with a longer 10-min re-exposure to the conditioned context (Fig. 2a–c), suggesting the presence of a time-limited refractory period for Arc expression in response to increasing context exposure, consistent with the findings of others22,23,24. The duration of recall is significant as a 2-min recall session does not appreciably affect conditioned freezing, but renders the CFM susceptible to disruption5, while 10-min recall produces extinction17. Infusing ARCASO into the dorsal hippocampus before SR resulted in a long-lasting decrease in fear memory (Fig. 3a), consistent with the proposed role of Arc in reconsolidation. This effect of the ARCASO was selective because the extinction associated with longer re-exposure was apparently unaffected by ARCASO. The efficacy of the ARCASO was confirmed by western blot by showing a sustained reduction of Arc protein expression in the CA1 following intrahippocampal ARCASO infusions (Supplementary Fig. 3). Furthermore, the decrease was confined to the CA1 and not to dorsal dg/CA3 samples from the same rats (Supplementary Fig. 4). This shows that the effects of the ARCASO were confined to the target site of the infusions and the ability of the ARCASO to disrupt the behavioural expression of fear memory is correlated with reduced Arc in CA1 specifically.
To differentiate the effects of ARCASO in either blocking reconsolidation or facilitating extinction with SR, we again used a brief reminder US. This manipulation revealed that after ARCASO treatment, the reminder US reinstated memory during recall (Fig. 3b). The reinstatement of the memory is seen when ARCASO or PBS is given before the SR (Fig. 3a), demonstrating that neither state-dependent retrieval deficits25 or new learning26 underlie the recovery of the CR. Therefore, the profile of ARCASO effects on the CR, like that of ZIFASO, is consistent with Arc playing a role in preventing extinction rather than in mediating reconsolidation. We additionally ruled out the requirement for the combined activities of Zif268 and Arc in reconsolidation via the co-infusion of ZIFASO and ARCASO before SR (Supplementary Fig. 5).
A key experimental underpinning of reconsolidation theory is the finding that blockade of protein synthesis following memory recall by anisomycin leads to a permanent amnesia4,5. This has been considered as proof that protein synthesis-dependent restabilization processes following recall-induced labilization are necessary for the memory to persist27. Using identical experimental manipulations to those we have used before5, we confirmed that anisomycin infused into the dorsal hippocampus after a SR trial produced the expected reduction in conditioned freezing at later test, consistent with previous studies (Fig. 4). However, we now show that the CFM can be subsequently recovered following the administration of a reminder US. The recovery of the CR after reminder is seen when anisomycin is additionally infused after the reminder US. This excludes the possibility that the initial reduction in CR derives from a retrieval failure via state-dependent mechanisms25, or is a result of new learning, strengthening or updating of the memory5,15. Anisomycin-treated animals also remain capable of fear learning in a new context, precluding any permanent damage to the hippocampus (Supplementary Fig. 6). Since a canonical characteristic of extinction is that the CR is temporarily suppressed but returns under various conditions, the reminder-induced return of CFM thus reveals an active, protein synthesis-dependent process in the hippocampus during SR that opposes extinction rather than mediating reconsolidation.
In conclusion, the observations that CFM can be recovered after using agents that are used routinely to define reconsolidation blockade calls us to reassess the phenomena of memory reconsolidation as a constructive, independent mnemonic process. Indeed, the temporary nature of CFM deficits by protein synthesis inhibitors at recall has been previously reported28,29. We add further, weight to these studies by showing recovery despite disrupting specific molecular correlates of reconsolidation. In addition these results are corroborated by human studies that have shown little evidence for the reconsolidation of hippocampal-dependent memories after retrieval30,31,32. Although we cannot exclude the possibility that reconsolidation may exist for other kinds of associative memory such as amygdala-based emotional memory4, a greater systematic investigative approach will be required in future when rejecting extinction-based interpretations in favour of reconsolidation-based views of recall-induced amnesia by different experimental interventions. A single example of memory rescue in such cases favours an extinction-based interpretation of the phenomena.
Our results suggest that when memory is recalled, a dynamic balance exists between maintaining the relevance of the original memory and extinction. We show that under conditions of short-term recall, Zif268- and Arc-dependent cellular processes in the hippocampus act to constrain extinction rather than to mediate reconsolidation. Under this model, changes in the expression of genes such as Zif268 and Arc in an early phase of recall blocks the premature engagement of extinction. Consequently, inhibiting the expression of these genes manifests as a more rapid extinction process that can then later be recovered by a reminder. However, under recall conditions that favour extinction, such as longer recall, these molecular inhibitors of extinction are inactive and extinction can proceed. As a consequence of identifying an active molecular process that prevents the extinction of memory, a simpler theoretical framework can be drawn on when assessing the site of action of treatments that influence memory expression; either by directly impacting on extinction or on the regulatory mechanisms that control extinction.
Reconsolidation is seen as an adaptive mechanism for maintaining memory relevance via updating or strengthening2. Furthermore, it is often held as a universal property of associative memories and failure to observe reconsolidation experimentally, termed ‘reconsolidation resistance’, have been explained away because the ‘boundary’ conditions to engage it were not exceeded, or were altered by experience via metaplastic processes at the network level33. In fact, the two key boundary conditions proposed for reconsolidation, the unpredictability of the retrieval stimulus34 and the presence of novel information35,36,37 are those that give rise to new learning including extinction7. Our experimental demonstration of reconsolidation blockade as facilitated extinction suggests that there is no competition between memory updating or strengthening (functions attributed to reconsolidation) and extinction, which are all described by similar learning rules, but rather it may suggest a molecular and cellular process that prevents new learning, which is gated by the similarity of the recalled memory to on-going experience38. In this regard, we can now focus on investigating the boundary conditions for extinction. Our data and the observation of the susceptibility of memory to disruption without retrieval (for example, refs 39, 40) are also more consistent with lingering consolidation, dynamic trace and comparator interpretations of memory41,42,43,44,45,46 rather than a recall-based reconsolidation view.
In summary, we demonstrate the recovery of contextual memories despite disrupting the molecular correlates of reconsolidation in the hippocampus. By doing so, we question the process of reconsolidation as a separate mnemonic memory process and suggest that the molecular events activated initially at recall act to constrain premature extinction. Instead, our results suggest that when memory is recalled, a dynamic balance exists between maintaining or strengthening the original memory and extinction.
The subjects were adult male Lister hooded rats weighing 280–350 g. They were housed in pairs, in holding rooms maintained at 21 °C on a reversed-light cycle (12-h light/dark; lights on at 2200 h). All experiments were conducted in the dark period of the rats. Food and water were freely available throughout the experiment. All procedures were conducted in accordance with local Cardiff University’s Animal Welfare and Ethical Review Body approval and the United Kingdom 1986 Animals (Scientific Procedures) Act (Project license PPLs 30/2236 and 30/2722). Sample size calculations were performed in http://www.stat.uiowa.edu/~rlenth/Power. All CFC rats were included in the final data analyses unless explicitly noted.
Surgery and microinfusions into the dorsal hippocampus
Steel double guide cannula aimed at the dorsal hippocampus (AP-3.50, relative to bregma) were surgically implanted under anaesthesia47 at least 1 week before behavioural training. Bilateral infusions via the chronically indwelling cannula were carried out in awake rats using a syringe pump, connected to injectors (28 gauge, projecting 1 mm beyond the guide cannula) by polyethylene tubing. Anisomycin (Sigma) was prepared at 80 μg μl−1 in sterile PBS (pH 7.4), while vehicle controls received PBS alone. ODNs were PAGE-purified phosphorothioate end-capped 18–20-mer sequences (SigmaGenosys): Arc antisense ODN, ARCASO, 5′-GTCCAGCTCCATCTGCTCGC-3′; Arc missense ODN, ARCMSO, 5′-CCACGCCATCGTGCCTTCGT-3′; Zif268 antisense ODN, ZIFASO, 5′-GGTAGTTGTCCATGGTGG-3′ and BDNF antisense ODN, BDNFASO, 5′-TCTTCCCCTTTTAATGGT-3′. All ODN sequences were subjected to a BLAST search on the National Center for Biotechnology Information BLAST server using the Genbank database. The antisense sequence had positive matches only for its target mRNA sequence, and no other rat or human-coding sequences. The control missense sequence, which included the same 20 nucleotides as the cogent ASO sequence but in a scrambled order, did not generate any full matches to identified gene sequences in the database. All ODNs were resuspended in sterile PBS (pH 7.4), ARCASO and ARCMSO to a concentration of 2 nmol μl−1, while combined ASO infusions gave a final concentration of each of 2 nmol μl−1. The volume of ODN infusions was 1 μl per hemisphere delivered at a rate of 0.125 μl min−1 and were infused 90 min before either recall or LTM1 sessions. The volume of anisomycin/vehicle infusions was 1 μl per hemisphere, delivered at a rate of 0.5 μl min−1 and given immediately after either recall or LTM1. All trained rats, unless indicated, were included in subsequent analyses. Where the procedure permitted, histological determination of the cannula placement using β-thionin staining of Nissl substance showed that all the brains examined showed the placement of cannula to be in the dorsal hippocampus with minimal tissue damage or ventricle enlargement.
Contextual fear conditioning
Conditioning was performed in one of two distinct contexts. During the 3-min conditioning training trial, rats received a single 2 s, 0.5 mA scrambled footshock (US) 2 min after being placed into the conditioning context. All rats were returned to the home cages after conditioning. Recall of CFM occurred 2–4 days later and consisted of exposing rats to the conditioned context for either 2 min (Short Recall, SR) or 10 min (Long Recall, longer re-exposure). Retrieval tests 3 h (post-retrieval short-term memory, PR-STM), or 2–39 days (long-term memory, LTM1- 3) after recall consisted of exposing the rat to the conditioning context for 2 min. In some experiments a 2 s, 0.25 mA scrambled footshock was given to co-terminate with the PR-STM or LTM1 as a reminder stimulus (Reminder US). Freezing behaviour served as a measure of the conditioned fear response (CR) to the context during the conditioning, extinction training and recall tests. This was video recorded and quantified by an observer blind to the experimental group. One unit of freezing was defined as a continuous absence of movement other than that required for respiration in 1 s sampled every 10 s. Freezing behaviour was analysed in a repeated measures analysis of variance (repeated measures ANOVA) with test as a within-subjects factor, or by ANOVA. For repeated measures ANOVA, Mauchly’s Test of Sphericity was applied. If the sphericity assumption was not met, the Greenhouse–Geisser correction was applied. Tukey’s test was then used for post hoc analysis to determine the sources of significance (*P<0.05, **P<0.01 and ***P<0.001). Post hoc planned comparisons were made using repeated measures ANOVA and the P-value constrained by the number of comparisons made.
In situ hybridization
Thirty minutes after recall rats were killed by carbon dioxide inhalation and the brain was rapidly removed and processed for in situ hybridization of an oligonucleotide probe (5′-AGCATCTCAGCTCGGCACTTACCAATCTGCAGGATCACATTGGGT-3′, SigmaGenosys Ltd, Cambridge, UK) complementary to nucleotides 288–332 of Arc (NM 019361), 3′-end-labelled with [α-35S] dATP17. Images were captured using LeicaQWIN imaging software (Leica Microsystems (UK) Ltd, Milton Keynes, UK) using an × 100 objective under oil immersion. Silver grain density was assessed in dorsal hippocampal CA1 pyramidal cells (approximately bregma −3.3 to 3.944 mm) using ImageJ imaging software ( http://rsbweb.nih.gov/ij/). Grains (total and non-specific) were counted over sufficient randomly selected neurons from each region for each animal such that the SE of the counts for any region was <10% of the population mean (typically 24 cells). In each case, cells were selected from at least three non-adjacent separate sections. A specific grain count was then calculated for each region by subtracting total and non-specific counts. The mean silver grain count in each region for each animal was then divided by the mean count in that region for the No Recall control group to give a standardized grain count (%) for each group. Standardized results were analysed by ANOVA, and post hoc comparisons were made using Tukey’s test.
At 2 or 6 h after retrieval testing, rats were sacrificed by carbon dioxide inhalation. The rats were decapitated and the brain was rapidly removed and placed on ice. The hippocampal dentate gyrus/CA3 and CA1 regions were microdissected and the dorsal half was isolated and frozen on dry ice before storage at −80 °C. Tissue lysates and western blotting were performed essentially as previously described47. Primary and secondary antibodies were diluted in TBST containing 0.5% Tween 20 and 2% ECL Advance blocking agent (GE Healthcare Inc, Chalfont St Giles, UK) and they were used at the following concentrations: Arc (H-300 Santa Cruz Biotechnology Inc, Insight Biotechnology Ltd, Wembley, UK), 1:20,000; GAPDH (ab9485, Abcam plc, Cambridge, UK), 1:10,000 and goat anti-rabbit IgG (whole-molecule)-peroxidase conjugate (Sigma-Aldrich Company Ltd. Poole, UK), 1:10,000. Incubation in antibody solutions were all for 1 h at room temperature with the exception of blots that were incubated with Arc antibody, which were incubated at 4 °C, overnight. Blots were visualized using ECL Advance detection (GE Healthcare Inc, Chalfont St Giles, UK) and opposed to autoradiographic film. Autoradiographs of each western blot were developed to be linear in the range used for densitometry for each protein target and for GAPDH. Autoradiographic images were scanned, digitized and the amounts of Arc and GAPDH signal were quantified for each sample using ImageJ ( http://rsbweb.nih.gov/ij). Averaging the amount of GAPDH across samples on each western blot and deriving a normalization factor for each sample corrected for loading variation. Levels of Arc protein were standardized with respect to the mean measured in the No Recall control group. ANOVA was applied and Tukey’s test was then used for post hoc analysis to determine the sources of significance.
How to cite this article: Trent, S. et al. Rescue of long-term memory after reconsolidation blockade. Nat. Commun. 6:7897 doi: 10.1038/ncomms8897 (2015).
Nader, K. & Hardt, O. A single standard for memory: the case for reconsolidation. Nat. Rev. Neurosci. 10, 224–234 (2009).
Lee, J. L. Reconsolidation: maintaining memory relevance. Trends Neurosci. 32, 413–420 (2009).
Misanin, J. R., Miller, R. R. & Lewis, D. J. Retrograde amnesia produced by electroconvulsive shock after reactivation of a consolidated memory trace. Science 160, 554–555 (1968).
Nader, K., Schafe, G. E. & Le Doux, J. E. Fear memories require protein synthesis in the amygdala for reconsolidation after retrieval. Nature 406, 722–726 (2000).
Lee, J. L., Everitt, B. J. & Thomas, K. L. Independent cellular processes for hippocampal memory consolidation and reconsolidation. Science 304, 839–843 (2004).
Pavlov, I. P. Conditioned reflexes: An investigation of the physiological activity of the cerebral cortex Oxford Univ. Press (1927).
Rescorla, R. A. & Wagner, A. R. A Theory of Pavlovian conditioning: Variations in the Effectiveness Of Reinforcement and Nonreinforcement Appleton-Century-Crofts (1972).
Bouton, M. E. Context, time, and memory retrieval in the interference paradigms of Pavlovian learning. Psychol. Bull. 114, 80–99 (1993).
Myers, K. M. & Davis, M. Mechanisms of fear extinction. Mol. Psychiatry 12, 120–150 (2007).
Eisenberg, M., Kobilo, T., Berman, D. E. & Dudai, Y. Stability of retrieved memory: inverse correlation with trace dominance. Science 301, 1102–1104 (2003).
Suzuki, A. et al. Memory reconsolidation and extinction have distinct temporal and biochemical signatures. J. Neurosci. 24, 4787–4795 (2004).
Cai, W. H., Blundell, J., Han, J., Greene, R. W. & Powell, C. M. Postreactivation glucocorticoids impair recall of established fear memory. J. Neurosci. 26, 9560–9566 (2006).
Blundell, J., Kouser, M. & Powell, C. M. Systemic inhibition of mammalian target of rapamycin inhibits fear memory reconsolidation. Neurobiol. Learn. Mem. 90, 28–35 (2008).
Hall, J., Thomas, K. L. & Everitt, B. J. Cellular imaging of zif268 expression in the hippocampus and amygdala during contextual and cued fear memory retrieval: selective activation of hippocampal CA1 neurons during the recall of contextual memories. J. Neurosci. 21, 2186–2193 (2001).
Lee, J. L. Memory reconsolidation mediates the strengthening of memories by additional learning. Nat. Neurosci. 11, 1264–1266 (2008).
Gold, P. E. & King, R. A. Retrograde amnesia: storage failure versus retrieval failure. Psychol. Rev. 81, 465–469 (1974).
Kirtley, A. & Thomas, K. L. The exclusive induction of extinction is gated by BDNF. Learn. Mem. 17, 612–619 (2010).
Maddox, S. A. & Schafe, G. E. The activity-regulated cytoskeletal-associated protein (Arc/Arg3.1) is required for reconsolidation of a Pavlovian fear memory. J. Neurosci. 31, 7073–7082 (2011).
Guzowski, J. F., Setlow, B., Wagner, E. K. & McGaugh, J. L. Experience-dependent gene expression in the rat hippocampus after spatial learning: a comparison of the immediate-early genes Arc, c-fos, and zif268. J. Neurosci. 21, 5089–5098 (2001).
Zhang, W. P., Guzowski, J. F. & Thomas, S. A. Mapping neuronal activation and the influence of adrenergic signaling during contextual memory retrieval. Learn. Mem. 12, 239–247 (2005).
Gusev, P. A., Cui, C., Alkon, D. L. & Gubin, A. N. Topography of Arc/Arg3.1 mRNA expression in the dorsal and ventral hippocampus induced by recent and remote spatial memory recall: dissociation of CA3 and CA1 activation. J. Neurosci. 25, 9384–9397 (2005).
Guzowski, J. F. et al. Recent behavioral history modifies coupling between cell activity and Arc gene transcription in hippocampal CA1 neurons. Proc. Natl Acad. Sci. USA 103, 1077–1082 (2006).
Mamiya, N. et al. Brain region-specific gene expression activation required for reconsolidation and extinction of contextual fear memory. J. Neurosci. 29, 402–413 (2009).
Maddox, S. A., Monsey, M. S. & Schafe, G. E. Early growth response gene 1 (Egr-1) is required for new and reactivated fear memories in the lateral amygdala. Learn. Mem. 18, 24–38 (2011).
Overton, D. A. State-dependent or "dissociated" learning produced with pentobarbital. J. Comp. Physiol. Psychol. 57, 3–12 (1964).
Guzowski, J. F. et al. Inhibition of activity-dependent arc protein expression in the rat hippocampus impairs the maintenance of long-term potentiation and the consolidation of long-term memory. J. Neurosci. 20, 3993–4001 (2000).
Ben Mamou, C., Gamache, K. & Nader, K. NMDA receptors are critical for unleashing consolidated auditory fear memories. Nat. Neurosci. 9, 1237–1239 (2006).
Lattal, K. M. & Abel, T. Behavioral impairments caused by injections of the protein synthesis inhibitor anisomycin after contextual retrieval reverse with time. Proc. Natl Acad. Sci. USA 101, 4667–4672 (2004).
Power, A. E., Berlau, D. J., McGaugh, J. L. & Steward, O. Anisomycin infused into the hippocampus fails to block "reconsolidation" but impairs extinction: the role of re-exposure duration. Learn. Mem. 13, 27–34 (2006).
Kindt, M., Soeter, M. & Vervliet, B. Beyond extinction: erasing human fear responses and preventing the return of fear. Nat. Neurosci. 12, 256–258 (2009).
Soeter, M. & Kindt, M. Dissociating response systems: erasing fear from memory. Neurobiol. Learn. Mem. 94, 30–41 (2010).
Soeter, M. & Kindt, M. Disrupting reconsolidation: pharmacological and behavioral manipulations. Learn. Mem. 18, 357–366 (2011).
Finnie, P. S. & Nader, K. The role of metaplasticity mechanisms in regulating memory destabilization and reconsolidation. Neurosci. Biobehav. Rev. 36, 1667–1707 (2012).
Dudai, Y. Reconsolidation: the advantage of being refocused. Curr. Opin. Neurobiol. 16, 174–178 (2006).
Pedreira, M. E., Perez-Cuesta, L. M. & Maldonado, H. Mismatch between what is expected and what actually occurs triggers memory reconsolidation or extinction. Learn. Mem. 11, 579–585 (2004).
Rodriguez-Ortiz, C. J., De la Cruz, V., Gutierrez, R. & Bermudez-Rattoni, F. Protein synthesis underlies post-retrieval memory consolidation to a restricted degree only when updated information is obtained. Learn. Mem. 12, 533–537 (2005).
Morris, R. G. et al. Memory reconsolidation: sensitivity of spatial memory to inhibition of protein synthesis in dorsal hippocampus during encoding and retrieval. Neuron 50, 479–489 (2006).
Osan, R., Tort, A. B. & Amaral, O. B. A mismatch-based model for memory reconsolidation and extinction in attractor networks. PLoS ONE 6, e23113 (2011).
Rodriguez-Ortiz, C. J., Balderas, I., Garcia-DeLaTorre, P. & Bermudez-Rattoni, F. Taste aversion memory reconsolidation is independent of its retrieval. Neurobiol. Learn. Mem. 98, 215–219 (2012).
Shema, R., Sacktor, T. C. & Dudai, Y. Rapid erasure of long-term memory associations in the cortex by an inhibitor of PKM zeta. Science 317, 951–953 (2007).
Dudai, Y. & Eisenberg, M. Rites of passage of the engram: reconsolidation and the lingering consolidation hypothesis. Neuron 44, 93–100 (2004).
Alberini, C. M. Mechanisms of memory stabilization: are consolidation and reconsolidation similar or distinct processes? Trends Neurosci. 28, 51–56 (2005).
McKenzie, S. & Eichenbaum, H. Consolidation and reconsolidation: two lives of memories? Neuron 71, 224–233 (2011).
Dudai, Y. & Morris, R. G. Memorable trends. Neuron 80, 742–750 (2013).
Hardt, O., Nader, K. & Nadel, L. Decay happens: the role of active forgetting in memory. Trends Cogn. Sci. 17, 111–120 (2013).
Stout, S. C. & Miller, R. R. Sometimes-competing retrieval (SOCR): a formalization of the comparator hypothesis. Psychol. Rev. 114, 759–783 (2007).
Barnes, P. & Thomas, K. L. Proteolysis of proBDNF is a key regulator in the formation of memory. PLoS ONE 3, e3248 (2008).
This work was supported by the BBSRC (BB/C00583X), a Wellcome Trust Institutional Strategic Support Fund (504182), a Wellcome Trust Strategic Award (503147) and a Medical Research Council Centre grant (G0801418). We acknowledge Professor Lawrence Wilkinson for his helpful comments.
The authors declare no competing financial interest.
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Trent, S., Barnes, P., Hall, J. et al. Rescue of long-term memory after reconsolidation blockade. Nat Commun 6, 7897 (2015). https://doi.org/10.1038/ncomms8897
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