Table 3 Antibodies and dyes used for histological evaluations.Abbreviation: NA=not applicable.

From: Loss of ATM accelerates pancreatic cancer formation and epithelial–mesenchymal transition

Antibodies: Company: Catalogue No. Species Dilution IHC Dilution IF
Primary antibodies
 Collagen 1 Abcam ab34710 Rabbit 1:500  
 Zeb1 Santa Cruz sc-25388 Rabbit 1:100 1: 2,000
 Sox9 Millipore AB5535 Rabbit 1:200 1: 8,000
 α-SMA Biozol AB5694 Rabbit 1:400  
 Amylase Sigma A8237 Rabbit 1:50 1:100
 N-cadherin Zymed 33-3900 Mouse 1:100  
 CK19 Abcam ab7755 mouse 1:100 1: 2,000
 Cyclin D1 Santa Cruz sc-753 Rabbit 1:50  
 Cyclin E Santa Cruz sc-481 Rabbit 1:200  
 BMP4 Santa Cruz sc-6896 Goat 1:200  
 Fibronectin Biomol A103 Rabbit 1:200  
 p53 Abcam ab26 Mouse 1:250  
 ATM Santa Cruz sc-7230 Rabbit 1:100  
 CPA1 R+D Systems AF2765 Goat 1:100 1: 5,000
 ki67 Dako M7249 Rat 1:100  
 CD45R BD 550286 Rat 1:50  
 Vimentin Max Plank Institute NA Mouse 1:50  
 CD133 Miltenyl Biotec 130-090-422 Mouse 1:20 1:20
 Ck8/18 Progen Biotechnik GP11 Guinea pig 1:200  
 RFP Abcam ab34771 Rabbit   1:100
Secondary antibodies
 Alexa Fluor 488 Life Technologies A-11001 Mouse   1:1,000
 Alexa Fluor 488 Life Technologies A-11008 Rabbit   1:1,000
 Alexa Fluor 568 Life Technologies A-10037 Mouse   1:1,000
 Alexa Fluor 568 Life Technologies A-11057 Goat   1:1,000
 Anti-guinea pig IgG (H+L) Vector BA7000   1:100  
 Anti-rabbit IgG (H+L) Vector BA-1000   1:100  
 Anti-mouse IgG (H+L) Vector BA-2000   1:100  
 Anti-goat IgG (H+L) Vector BA-5000   1:100  
Dyes Company: Catalogue No.
Tunel Calbiochem Q1A33
Masson Goldner Merck 100485
Alcian blue NA NA