Signal transduction in living cells is vital to maintain life itself, where information transfer in noisy environment plays a significant role. In a rather different context, the recent intensive research on ‘Maxwell's demon’—a feedback controller that utilizes information of individual molecules—have led to a unified theory of information and thermodynamics. Here we combine these two streams of research, and show that the second law of thermodynamics with information reveals the fundamental limit of the robustness of signal transduction against environmental fluctuations. Especially, we find that the degree of robustness is quantitatively characterized by an informational quantity called transfer entropy. Our information-thermodynamic approach is applicable to biological communication inside cells, in which there is no explicit channel coding in contrast to artificial communication. Our result could open up a novel biophysical approach to understand information processing in living systems on the basis of the fundamental information–thermodynamics link.
A crucial feature of biological signal transduction lies in the fact that it works in noisy environment1,2,3. To understand its mechanism, signal transduction has been modelled as noisy information processing4,5,6,7,8,9,10,11. For example, signal transduction of bacterial chemotaxis of Escherichia coli (E. coli) has been investigated as a simple model organism for sensory adaptation12,13,14,15,16. A crucial ingredient of E. coli chemotaxis is a feedback loop, which enhances the robustness of the signal transduction against environmental noise.
The information transmission inside the feedback loop can be quantified by the transfer entropy, which was originally introduced in the context of time series analysis17, and has been studied in electrophysiological systems18, chemical processes19 and artificial sensorimotors20. The transfer entropy is the conditional mutual information representing the directed information flow, and gives an upper bound of the redundancy of the channel coding in an artificial communication channel with a feedback loop21; this is a fundamental consequence of Shannon’s second theorem22,23. However, as there is not any explicit channel coding inside living cells, the role of the transfer entropy in biological communication has not been fully understood.
The transfer entropy also plays a significant role in thermodynamics24. Historically, the connection between thermodynamics and information was first discussed in the thought experiment of ‘Maxwell’s demon’ in the nineteenth century25,26,27, where the demon is regarded as a feedback controller. In the recent progress on this problem in light of modern non-equilibrium statistical physics28,29, a universal and quantitative theory of thermodynamics feedback control has been developed, leading to the field of information thermodynamics24,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48. Information thermodynamics reveals a generalization of the second law of thermodynamics, which implies that the entropy production of a target system is bounded by the transfer entropy from the target system to the outside world24.
In this article, we apply the generalized second law to establish the quantitative relationship between the transfer entropy and the robustness of adaptive signal transduction against noise. We show that the transfer entropy gives the fundamental upper bound of the robustness, elucidating an analogy between information thermodynamics and the Shannon’s information theory22,23. We numerically studied the information-thermodynamics efficiency of the signal transduction of E. coli chemotaxis, and found that the signal transduction of E. coli chemotaxis is efficient as an information-thermodynamic device, even when it is highly dissipative as a conventional heat engine.
The main components of E. coli chemotaxis are the ligand density change l, the kinase activity a and the methylation level m of the receptor (Fig. 1). A feedback loop exists between a and m, which reduces the environmental noise in the signal transduction pathway from l to a (ref. 49). Let lt, at and mt be the values of these quantities at time t. They obey stochastic dynamics due to the noise, and are described by the the following coupled Langevin equations7,14,16:
where is the stationary value of the kinase activity under the instantaneous values of the methylation level mt and the ligand signal lt. In the case of E. coli chemotaxis, we can approximate as αmt−βlt, by linearizing it around the steady-state value7,14. (x = a,m) is the white Gaussian noise with and , where 〈⋯〉 describes the ensemble average. describes the intensity of the environmental noise at time t, which is not necessarily thermal inside cells. The noise intensity characterizes the ligand fluctuation. The time constants satisfy , which implies that the relaxation of a to is much faster than that of m.
The mechanism of adaptation in this model is as follows (Fig. 2; refs 14, 16). Suppose that the system is initially in a stationary state with lt = 0 and at time t < 0, and lt suddenly changes from 0 to 1 at time t = 0 as a step function. Then, at rapidly equilibrates to so that the difference becomes small. The difference plays an important role, which characterizes the level of adaptation. Next, mt gradually changes to satisfy , and thus at returns to 0, where remains small.
Robustness against environmental noise
We introduce a key quantity that characterizes the robustness of adaptation, which is defined as the difference between the intensity of the ligand noise and the mean square error of the level of adaptation :
The larger is, the more robust the signal transduction is against the environmental noise. In the case of thermodynamics, corresponds to the heat absorption in a and characterizes the violation of the fluctuation–dissipation theorem28. Since the environmental noise is not necessarily thermal in the present situation, is not exactly the same as the heat, but is a biophysical quantity that characterizes the robustness of adaptation against the environmental noise.
We here discuss the quantitative definition of the transfer entropy17. The transfer entropy from a to m at time t is defined as the conditional mutual information between at and mt+dt under the condition of mt:
where p[mt+dt,at,mt] is the joint probability distribution of (mt+dt,at,mt), and p[mt+dt|at,mt] is the probability distribution of mt+dt under the condition of (at,mt). The transfer entropy characterizes the directed information flow from a to m during an infinitesimal time interval dt (refs 17, 50), which quantifies a causal influence between them51,52. From the non-negativity of the conditional mutual information23, that of the transfer entropy follows: .
Second law of information thermodynamics
We now consider the second law of information thermodynamics, which characterizes the entropy change in a subsystem in terms of the information flow (Fig. 3). In the case of equation (1), the generalized second law is given as follows (see also Methods section):
Here, is the conditional Shannon entropy change defined as with , which vanishes in the stationary state. The transfer entropy dIttr on the left-hand side of equation (4) shows the significant role of the feedback loop, implying that the robustness of adaptation can be enhanced against the environmental noise by the feedback using information. This is analogous to the central feature of Maxwell’s demon.
To further clarify the meaning of inequality (equation (4)), we focus on the case of the stationary state. If there was no feedback loop between m and a, then the second law reduces to , which, as naturally expected, implies that the fluctuation of the signal transduction is bounded by the intensity of the environmental noise. In contrast, in the presence of a feedback loop, can be smaller than owing to the transfer entropy in the feedback loop:
This inequality clarifies the role of the transfer entropy in biochemical signal transduction; the transfer entropy characterizes an upper bound of the robustness of the signal transduction in the biochemical network. The equality in equation (5) is achieved in the limit of α → 0 and τa/τm → 0 for the linear case with (Supplementary Note 1). The latter limit means that a relaxes infinitely fast and the process is quasi-static (that is, reversible) in terms of a. This is analogous to the fact that Maxwell’s demon can achieve the maximum thermodynamics gain in reversible processes35. In general, the information-thermodynamic bound becomes tight if α and τm/τa are both small. The realistic parameters of the bacterial chemotaxis are given by and (refs 7, 14, 16), and therefore the real adaptation process is accompanied by a finite amount of information-thermodynamics dissipation.
Our model of chemotaxis has the same mathematical structure as the feedback cooling of a colloidal particle by Maxwell’s demon36,38,42,47, where the feedback cooling is analogous to the noise filtering in the sensory adaptation49. This analogy is a central idea of our study; the information-thermodynamic inequalities (equation (5) in our case) characterize the robustness of adaptation as well as the performance of feedback cooling.
We consider the second law (equation (4)) in non-stationary dynamics, and numerically demonstrate the power of this inequality. Figure 4 shows and
in six different types of dynamics of adaptation, where the ligand signal is given by a step function (Fig. 4a), a sinusoidal function (Fig. 4b), a linear function (Fig. 4c), an exponential decay (Fig. 4d), a square wave (Fig. 4e) and a triangle wave (Fig. 4f). These results confirm that gives a tight bound of , implying that the transfer entropy characterizes the robustness well. In Fig. 4b,f, the robustness is nearly equal to the information-thermodynamics bound when the signal and noise are decreasing or increasing rapidly (for example, and t = 0.012 in Fig. 4f).
Conventional second law of thermodynamics
For the purpose of comparison, we next consider another upper bound of the robustness, which is given by the conventional second law of thermodynamics without information. We define the heat absorption by m as , and the Shannon entropy change in the total system as with , which vanishes in the stationary state. We can then show that
is an upper bound of , as a straightforward consequence of the conventional second law of thermodynamics of the total system of a and m (refs 28, 29). The conventional second law implies that the dissipation in m should compensate for that in a (Fig. 3). Figure 4 shows along with and . Remarkably, information-thermodynamic bound gives a tighter bound of than the conventional thermodynamics bound such that
To compare the information-thermodynamic bound and the conventional thermodynamics one more quantitatively, we introduce an information-thermodynamic figure of merit based on the inequalities (equation (8)):
where the second term on the right-hand side is given by the ratio between the information-thermodynamic dissipation and the entire thermodynamic dissipation . This quantity satisfies 0 ≤ χ ≤ 1, and () means that information-thermodynamic bound is much tighter (a little tighter) compared with the conventional thermodynamic bound. We numerically calculated χ in the aforementioned six types of dynamics of adaptation (Supplementary Figs 1–6). In the case of a linear function (Supplementary Fig. 3), we found that χ increases in time t and approaches to . In this case, the signal transduction of E. coli chemotaxis is highly dissipative as a thermodynamic engine, but efficient as an information transmission device.
Comparison with Shannon’s theory
We here discuss the similarity and the difference between our result and the Shannon’s information theory (refs 22, 23; Fig. 5). The Shannon’s second theorem (that is, the noisy-channel coding theorem) states that an upper bound of achievable information rate R is given by the channel capacity C such that C ≥ R. The channel capacity C is defined as the maximum value of the mutual information with finite power, where the mutual information can be replaced by the transfer entropy in the presence of a feedback loop21. R describes how long bit sequence is needed for a channel coding to realize errorless communication through a noisy channel, where errorless means the coincidence between the input and output messages. Therefore, both of and R characterize the robustness information transmission against noise, and bounded by the transfer entropy . In this sense, there exists an analogy between the second law of thermodynamics with information and the Shannon’s second theorem. In the case of biochemical signal transduction, the information-thermodynamic approach is more relevant, because there is not any explicit channel coding inside cells. Moreover, while is an experimentally measurable quantity as mentioned below 28,29, R cannot be properly defined in the absence of any artificial channel coding 23. Therefore, is an intrinsic quantity to characterize the robustness of the information transduction inside cells.
Our result can be experimentally validated by measuring the transfer entropy and thermodynamics quantities from the probability distribution of the amount of proteins in a biochemical system5,6,9,10,46,47,48,49. In fact, the transfer entropy dItr and thermodynamics quantities (that is, and ) can be obtained from the joint probability distribution of (at,mt,at+dt,mt+dt). The measurement of such a joint distribution would not be far from today’s experimental technique in biophysics5,6,9,10,53,54,55,56. Experimental measurements of and would lead to a novel classification of signal transduction in terms of the thermodynamics cost of information transmission.
We note that, in ref. 16, the authors discussed that the entropy changes in two heat baths can be characterized by the accuracy of adaptation. In our study, we derived a bound for that is regarded as the robustness of signal transduction against the environmental noise. These two results capture complementary aspects of adaptation processes: accuracy and robustness.
We also note that our theory of information thermodynamics24 can be generalized to a broad class of signal transduction networks, including a feedback loop with time delay.
The outline of the derivation of inequality (4)
We here show the outline of the derivation of the information-thermodynamic inequality (equation (4); see also Supplementary Note 2 for details). The heat dissipation is given by the ratio between forward and backward path probabilities as (refs 24, 28, 29), where the backward path probability can be calculated from the forward path probability . Thus, the difference is given by the Kullback–Libler divergence23. From its non-negativity23, we have . This inequality can be derived from the general inequality of information thermodynamics24 (see Supplementary Note 3 and Supplementary Fig. 7). As discussed in Supplementary Note 3, this inequality gives a weaker bound of the entropy production.
The analytical expression of the transfer entropy
In the case of E. coli chemotaxis, we have , and equation (1) become linear. In this situation, if the initial distribution is Gaussian, we analytically obtain the transfer entropy up to the order of dt (Supplementary Note 4): , where describes the intensity of the environmental noise, and describes the intensity of the signal from a to m per unit time with , and . We note that dItr for the Gaussian case is greater than that of the non-Gaussian case, if and are the same23. We also note that the above analytical expression of is the same form as the Shannon–Hartley theorem23.
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We are grateful to S.-I. Sasa, U. Seifert, M. L. Rosinberg, N. Shiraishi, K. Kawaguchi, H. Tajima, A.C. Barato, D. Hartich and M. Sano for their valuable discussions. This work was supported by the Grants-in-Aid for JSPS Fellows (grant no. 24·8593), by JSPS KAKENHI grant numbers 25800217 and 22340114, by KAKENHI no. 25103003 ‘Fluctuation & Structure’ and by Platform for Dynamic Approaches to Living System from MEXT, Japan.
The authors declare no competing financial interests.
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Ito, S., Sagawa, T. Maxwell’s demon in biochemical signal transduction with feedback loop. Nat Commun 6, 7498 (2015). https://doi.org/10.1038/ncomms8498
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