Figure 1 : AO correction via direct wavefront sensing improves morphological imaging deep inside the cortex of a living mouse.

From: Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Figure 1

(a) 120 × 120 μm field-of-view single-plane TPE fluorescence images of neurons in a Thy1-YFPH mouse at 600–620 μm below pia after AO correction. Scale bar, 20 μm. (b) TPE fluorescence images of dendrites at 606, 606.5 and 608.5-μm depth taken with objective correction ring adjustment only (left) and correction ring adjustment plus AO (right). Scale bar, 5 μm. (c) SH sensor image (left) for an NIR GS produced by TPE excitation of directly injected ICG, and the corresponding corrective wavefront (right). Representative images from >500 image sections in five mice.