Figure 3: Ileectomy and knockdown of hepatic Fgfr4 restore Cyp7a1 expression and BA synthesis in Klf15−/− mice. | Nature Communications

Figure 3: Ileectomy and knockdown of hepatic Fgfr4 restore Cyp7a1 expression and BA synthesis in Klf15−/− mice.

From: Circadian control of bile acid synthesis by a KLF15-Fgf15 axis

Figure 3

(a) Schematic picture before and after ileectomy (IleΔ). Approximately 90% of the ileum was resected. (b) FGF15 protein concentration (left) and total amount (right) in blood from Klf15+/+ and Klf15−/− mice at ZT14 after sham or IleΔ surgery (n=10). (c) The mRNA levels of hepatic Cyp7a1 in Klf15+/+ and Klf15−/− mice at ZT14 after sham or IleΔ (n=5). (d) BA amount in liver, intestine (Int) and GB in Klf15+/+ versus Klf15−/−, sham versus IleΔ mice (n=5). (e) mRNA expression of the liver Cyp7a1 and Fgfr4 in Klf15+/+ and Klf15−/− mice infected with adenoviral shRNA control (Ctl) or shFgfr4 (n=5). Statistical significance between the two individual groups was assessed using Student’s t-test. Normal distribution was assumed. * indicates P<0.05, compared with Klf15+/+ mice with sham surgery or infected with Ctl.

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