Table 1 Data collection and refinement statistics for injectisome structures.

From: A common assembly module in injectisome and flagellar type III secretion sorting platforms

  SpaO(232–297, SeMet) SpaO(145–213, SeMet)+SpaO(232–297, SeMet) SpaO(145–213)+SpaO(232–297) SpaO(145–213)+SpaO(232–297)+OrgB(1–30)::lysozyme SpaO(145–213, SeMet)+SpaO(232–297, SeMet)+OrgB(1–30)::lysozyme
Data collection
 Space group P 21 P 41 21 2 P 41 21 2 P 21 P 21
 Cell dimensions
a, b, c (Å) 35, 41.27, 48 66.38, 66.38, 95.21 65.76, 65.76, 95.65 62.092, 89.07, 62.092 62.88, 88.5, 63.32
α, β, γ (°) 90, 103.92, 90 90, 90, 90 90, 90, 90 90, 114.94, 90 90, 116.07, 90
 Resolution (Å) 31.26–1.35 (1.37–1.35) 46.94–3.00 (3.18–3.00) 38.68–2.9 (3.08–2.9) 47.59–2.0 (2.05–2.0) 45.8–2.35 (2.43–2.35)
Rmerge 0.146 (1.281) 0.221 (1.463) 0.166 (1.447) 0.102 (0.530) 0.088 (0.617)
II 8.6 (2.1) 11.8 (3.1) 14.4 (2.7) 10.5 (3.2) 12.8 (2.6)
 CC1/2 0.994 (0.750) 0.989 (0.839) 0.996 (0.856) 0.994 (0.828) 0.997 (0.816)
 Completeness (%) 99.7 (100) 100 (100) 99.3 (99.3) 99.5 (99.8) 99.0 (99.6)
 Redundancy 7.0 (7.1) 25.6 (27.2) 24.6 (26.2) 5.1 (5.2) 6.6 (6.6)
Refinement
 No. of reflections 29,246   4,964 41,183 25,740
Rwork/Rfree 0.1724/0.2053   0.2085/0.2795 0.1571/0.2096 0.1984/0.2618
 No. of atoms 1,286   1,024 5,769 4,940
Protein 1,062   1,023 5,112 4,818
Ligand/ion 2   1 0 0
Water 222   0 657 122
B factors
Protein 14.70   74.20 33.10 46.90
Ligand/ion 14.20   105.00   
Water 32.10    39.60 45.60
 r.m.s.d.
Bond lengths (Å) 0.007   0.010 0.008 0.011
Bond angles (°) 1.09   1.33 1.16 1.46
  1. SeMet, selenomethionine.