(a) Approximately 650 genes were examined in the fat body, 61 whose loss in the fat body resulted in significant hyperglycaemia; statistical significance was evaluated as outlined in Fig. 3. n=4 each (⩾10 larvae per replicate). Statistics were initially assessed by two-tailed Student’s t-test (P<0.05, error bars indicate s.e.m.). Hits were reclassified based on P values from the Wilcoxon rank-sum test. High confidence (in magenta): P<0.05, medium confidence (in blue): P=0.057 and low confidence (in grey): P>0.057. Genes are alphabetically listed in each confidence level. After the primary screen, three repeat glucose fold changes for hyperglycaemia candidates were assessed by a 95% confidence interval; Ф symbol above bar indicates the genes not validated with 95% confidence. (b) Number of primary screen hits linked to glucose regulation and novel hits are indicated. (c) Classification of candidates obtained in all RNAi-glucose screens. Diab. cand., diabetes candidate;Gluc. met., glucose metabolism.