Figure 3: Tissue-specific Glucome RNAi screening of 95 kinases. | Nature Communications

Figure 3: Tissue-specific Glucome RNAi screening of 95 kinases.

From: Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism

Figure 3

(a,b) Primary screening, using quadruplicate independent samples, of 95 kinase genes produced significant glucose elevations for 24 genes in the fat body (a) and 27 genes in the muscle (b); n=4 each (10 larvae per replicate), statistical significance was initially assessed by two-tailed Student’s t-test (P<0.05, error bars indicate s.e.m.). # indicates a common hit in fat body and muscle screens. Student’s t-test-positive (P<0.05) ‘hits’ were reclassified based on P values from the Wilcoxon rank-sum test. High confidence to left and in magenta: P<0.05, medium confidence toward right and in blue: P=0.057, and low confidence at right end and in grey: P>0.057. Genes are alphabetically listed in each confidence level. After the primary screen, at least three repeat independent glucose measurements, in quadruplicate, were done for each hyperglycaemia candidate identified in the t-test/Wilcoxon test evaluations and then assessed by a binomial 95% confidence interval evaluations; Ф symbol above bar indicates the very few genes not validated with at least 95% confidence in the binomial evaluations of the three independent repetitions. (c) The proportion of hyperglycaemia kinase hits from primary screen in either tissue, overlapping hits in both tissues, and genes previously associated with glucose metabolism or diabetes and novel genes are indicated.

Back to article page