Figure 3 : Interactions of the MifM nascent chain with components of the ribosomal tunnel.

From: Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling

Figure 3

(af) Contacts between MifM nascent chain (green) and the ribosome in the (ac) upper and (df) lower region of the ribosomal tunnel. Electron density (mesh) is coloured for MifM (green), L4 (cyan), L22 (orange) and rRNA (grey). In (df) the cryo-EM map was filtered to 4 Å resolution and the nascent chain modelled as a backbone trace. (g) Schematic for the GFP–MifM–LacZ reporter used to monitor translational arrest, where stalling prevents β-galactosidase production in B. subtilis in vivo. (hj) β-galactosidase activity from the GFP–MifM–LacZ reporter (as in g) expressed in B. subtilis strains bearing wildtype (WT) or truncated versions of ribosomal proteins (h) L4, (i) L22 or (j) L23. Error bars indicate the s.d. of three independent biological replicates.