Figure 7 : Neurons express high levels of HK2 and 3.

From: Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism

Figure 7

(a) Immunohistochemistry of HK2 (red) in neurons (NeuN+, grey arrow) and astrocytes (green arrow, eGFP+) with DAPI (blue) of a GLT1-eGFP reporter mouse from the surface of cortex through to the corpus callosum. Scale bar, 50 μm. (b) HK2 (red) and NeuN (white) immunohistochemistry in GLT1-eGFP mouse cortex. Scale bar, 50 μm. (c) Percentage difference in HK2 immunolabelling intensity relative to cell-free parenchyma in the cytosol of NeuN+ neurons (grey) and GLT1-eGFP+ astrocytes (green) in the cortex (P<0.001), striatum (P<0.05), CA1 (P<0.05) and CA3 (P<0.01), t-test (30–50 neurons and 30–50 astrocytes in N=4 mice). (d) HK3 (red) and NeuN (white) immunohistochemistry in GLT1-eGFP mouse cortex. Scale bar, 50 μm. (e) Percentage difference in HK3 immunolabelling intensity relative to cell-free parenchyma in the cytosol of NeuN+ neurons (grey) and GLT1-eGFP+ astrocytes (green) in the cortex, striatum, CA1 and CA3. P<0.01, t-test (35–50 neurons and 35–50 astrocytes in N=4 mice). Bar graphs represent mean±s.e.m.