Figure 6 : Neurons express high levels of hexokinases.

From: Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism

Figure 6

(a) Left: diagram of FACS sorting strategy for qPCR of hK1-3. Right: qPCR analysis of hK1–3 expression in FACS-sorted cortical neurons (tdTomato+ versus tdTomato cell population harvested from Camk2a-CreERT2/CAG-tdTomato reporter mice) and astrocytes (eGFP+ versus eGFP cell populations harvested from GLT1-eGFP reporter mice) expressed as log2 of the ratio of positive versus negative population. P<0.05 compared with negative populations for hK1–3 in neurons and hK3 in astrocytes (N=3 mice in each group). (b,c) Immunohistochemistry of HK1 (red) in neurons (NeuN+, grey arrow) and astrocytes (green arrow, eGFP+) with DAPI (blue) in the cortex of a GLT1-eGFP reporter mouse. Scale bar, 50 μm. (d) Orthogonal projections of a 0.1-μm step size Z-stack. Green arrow indicates an astrocyte endfoot. (e) Quantification of HK1 immunoflourescence shown in percentage difference in HK1 immunolabelling intensity relative to cell-free parenchyma in the cytosol of NeuN+ neurons (grey) and GLT1-eGFP+ astrocytes (green) in the cortex. P<0.01, t-test (40 neurons and 40 astrocytes from N=4 mice). (fh) Immunohistochemistry of HK1 and NeuN in GLT1-eGFP mouse and (ik) percentage difference in immunolabelling intensity relative to cell-free parenchyma in the striatum, CA1 and CA3, respectively. Scale bars, 50 μm (fh); P<0.001 for the striatum, P<0.05 for CA1 and CA3, t-test (40 neurons and 40 astrocytes in N=4 mice). Bar graphs represent mean±s.e.m.