Figure 5 : Functional activation increases neuronal uptake, but not astrocytic uptake of 2DG-IR.

From: Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism

Figure 5

(a) Upper panel: diagram of experimental setup. Whisker stimulation (air puffs, 3 Hz, 30 min) induced an increase in uptake of 2DG-IR and 14C-2DG in contralateral somatosensory barrel cortex (S1BF). Lower panel: LFP recording in contralateral cortex 1 min before, 1, 15 and 29 min into the stimulation and 1 min after whisker stimulation. (b) Representative images of 2DG-IR fluorescence and (c) 14C-2DG autoradiography of whisker stimulated mice. White dotted lines outline hippocampus. Contralateral cortex was 19±4% higher than ipsilateral cortex. P<0.01, paired t test (N=5). (d) Quantification of 2DG-IR fluorescence in contralateral and ipsilateral barrel cortex in mice with whisker stimulation. P=0.003, paired t-test (N=7). Representative images of 2DG-IR uptake in neurons and astrocytes in (e) ipsilateral and (f) contralateral cortex of whisker stimulated mice. Scale bars, 20 μm. Right panel: close-up images of 2DGIR in neurons and astrocytes in ipsi- and contralateral cortex with and without eGFP and yellow-Hoechst signals. (g) Quantification of 2DG-IR fluorescence in neurons relative to astrocytes in the respective regions. P=0.006, t test (N=7 contralateral and N=9 ipsilateral). Bar graphs represent mean±s.e.m.