Figure 1 : Preferential neuronal uptake of glucose and glucose analogues in vitro.

From: Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism

Figure 1

(a) Immunocytochemistry of NeuN (grey), GFAP (red) and DAPI (blue) in neuronal cultures from wild-type mice (left) and astrocyte cultures from GLT1-eGFP mice (right). Insert: GFAP (left) and NeuN (right) staining signals alone showed no staining signals. Scale bar, 50 μm. (b) 14C-glucose uptake, and (c) 14C-2DG and 2DG-IR uptake in neurons and astrocytes plotted as a function of time. Slopes for initial linear phase 14C-glucose neurons, 1.15±0.06; 14C-glucose astrocytes, 0.20±0.01; 14C-2DG neurons, 1.39±0.03; 14C-2DG astrocytes, 0.23 ±0.01 (nmol per mg protein min−1); 2DG-IR neurons, 245.00±30.52; 2DG-IR astrocytes, 28.17±1.41 (in arbitrary units, au min−1). (d) Substrate competition of 14C-2DG and 2DG-IR uptake plotted as a function of increasing concentration of D-glucose (Ki in mM: 14C-2DG neurons, 0.41±0.01; 2DG-IR neurons, 0.87±0.04; 14C-2DG astrocytes, 0.71±0.0). (e) Effect of increasing concentrations of the nonspecific glucose transporter inhibitor, cytochalasin B, on 14C-glucose uptake (Ki in μM: 14C-glucose neurons, 2.58±0.11; 14C-glucose astrocytes, 4.39±0.03). (f) The effect of cytochalasin B effect on 14C-2DG and 2DG-IR uptake (Ki in μM: 14C-2DG neurons, 2.59±0.04; 2DG-IR neurons, 3.02±0.23; 14C-2DG astrocytes, 4.64±0.06; 2DG-IR astrocytes, 1.66±0.06). The cultures were pretreated with cytochalasin B for 10 min before addition of 14C-glucose, 14C-2DG, or 2DG-IR. All values are expressed as mean±s.e.m.