Figure 4 : Subcellular localization of ERK7 in MCCs.

From: ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled

Figure 4

(a) Subcellular localization of ERK7-EGFP in the epidermis. ERK7-EGFP mRNA (25 pg per cell) was injected into the animal region of four blastomeres at the four-cell stage, and the injected embryos were observed at stage 33/34. (b) Subcellular localization of ERK7-EGFP (green) and Centrin2-mCherry (red) in the MCCs. ERK7-EGFP mRNA (25 pg per cell) and Centrin2-mCherry mRNA (50 pg per cell) were injected into the ventral marginal region of two blastomeres at the four-cell stage, and the injected embryos were observed at stage 35/36. (c) Subcellular localization of ERK7-EGFP (green) and CLAMP-mCherry (red) in the MCCs. ERK7-EGFP mRNA (25 pg per cell) and CLAMP-mCherry mRNA (100 pg per cell) were injected into the ventral two blastomeres at the four-cell stage, and the injected embryos were fixed at stage 33/34 and observed. Data are representative of two independent experiments. Scale bars, 5 μm.