Figure 2 : ERK7 is required for ciliogenesis.

From: ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled

Figure 2

(a) The trajectories of fluorescent beads are shown in a colour gradient (see colour bar). Each MO (10 ng per cell) was injected into the animal region of four blastomeres at the four-cell stage. White lines indicate an outline of the embryo. See Supplementary Movies 3 and 4. Scale bars, 300 μm. (b) The flow velocity of the beads was measured using ImageJ. Error bars represent the s.d. of 90 beads from nine embryo movies for each condition. ***P<0.001 by the t-test. (ce) Immunostaining with anti-acetylated-α-tubulin (Ac.-tub) to visualize cilia (green). As a tracer, membrane-targeted (mem)-mCherry (red) was used. (c,d) Each MO (10 ng per cell) was injected into the animal region of four blastomeres at the four-cell stage. The injected embryos were fixed at stage 35/36. (c) Whole embryos. Scale bars, 300 μm. (d) Multicilia on MCCs. Scale bars, 5 μm. (e) Cilia on the GRP. Each MO (15 ng/cell) was injected into the dorsal marginal region of two blastomeres at the four-cell stage. The right graph shows the average length of cilia on the GRP (control MO, n=62 from five dorsal explants; ERK7 MO, n=97 from five dorsal explants). Error bars represent the s.d. Scale bars, 5 μm. ***P<0.001 by the t-test.