Figure 5: Levels of LINE-1 RNA and genomic insertions increase in mice with loss of Becn1/Atg6. | Nature Communications

Figure 5: Levels of LINE-1 RNA and genomic insertions increase in mice with loss of Becn1/Atg6.

From: Autophagy supports genomic stability by degrading retrotransposon RNA

Figure 5

(a) RT-qPCR analysis of the levels of LINE-1 ORF1 and ORF2 RNA in ovaries of Becn1/Atg6+/− mice compared with wild-type controls (n=4 wild-type, Becn1/Atg6+/− mice). (b) qPCR analysis of relative number of genomic copies of LINE-1 ORF2 in ovaries of Becn1/Atg6+/− mice and wild-type controls normalized to LINE-1 5′UTR and 5S rDNA (n=4 wild-type, 6 Becn1/Atg6+/− mice). *P=0.02, t-test. (c) RT-qPCR analysis of the levels of LINE-1 ORF1 and ORF2 RNA in the prefrontal cortex of Becn1/Atg6+/− mice compared with wild-type controls (n=4 wild-type, Becn1/Atg6+/− mice). (d) qPCR analysis of relative number of genomic copies of LINE-1 ORF2 in muscle, liver and prefrontal cortex of Becn1/Atg6+/− mice and wild-type controls normalized to LINE-1 5′UTR and 5S rDNA (n=4 wild-type, 6 Becn1/Atg6+/− mice). *P=0.019 prefrontal cortex, P=0.048 muscle, analysis of variance. All error bars represent s.e. of the mean.

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