Control over interactions with biomolecules holds the key to applications of graphene in biotechnology. One such application is nanopore sequencing, where a DNA molecule is electrophoretically driven through a graphene nanopore. Here we investigate how interactions of single-stranded DNA and a graphene membrane can be controlled by electrically biasing the membrane. The results of our molecular dynamics simulations suggest that electric charge on graphene can force a DNA homopolymer to adopt a range of strikingly different conformations. The conformational response is sensitive to even very subtle nucleotide modifications, such as DNA methylation. The speed of DNA motion through a graphene nanopore is strongly affected by the graphene charge: a positive charge accelerates the motion, whereas a negative charge arrests it. As a possible application of the effect, we demonstrate stop-and-go transport of DNA controlled by the charge of graphene. Such on-demand transport of DNA is essential for realizing nanopore sequencing.
Control over the interactions between biomolecules and carbon-based materials may hold the key to unlocking potential of nanotechnology for biomedical applications1. One such application is nanopore sequencing of DNA, which may enable ultra-rapid sequencing of human genomes at a fraction of the cost of current generation DNA-sequencing methods2,3,4. In nanopore sequencing, single-stranded DNA (ssDNA) is electrophoretically driven through a nanopore embedded in a nanometer thin membrane5. As ssDNA transits the nanopore, individual DNA nucleotides affect the ability of dissolved ions to pass through the nanopore. Thus, a measurement of nanopore ionic current can provide, in principle, a real-time readout of the DNA nucleotide sequence. Among the advantages of nanopore sequencing are the potentially limitless read lengths, minimal requirements for consumable reagents, the ability to read native DNA and re-read the same fragment of a DNA molecule multiple times6,7.
Graphene, a single layer of hexagonally arranged carbon atoms8, harbours unique electrical and physical properties conducive to nanopore-based DNA sequencing. The atomically thin structure of graphene and its straightforward layerability allows the membrane thickness to be precisely controlled at the scale comparable to the distance between neighbouring nucleotides in a DNA strand. Recent advances in transmission electron microscopy9, developing lithography10 and bottom-up growth techniques11,12,13 have enabled atomic-scale manufacturing of graphene-based nanostructures. Furthermore, the unique electrical properties of graphene offer, at least in theory, several plausible methods for DNA sequence detection14, including ionic current readout15,16, nanoribbon conductance17,18,19,20 and transverse tunnelling21,22,23.
Several experimental groups have reported measurements of ionic current signals produced by interactions of double-stranded DNA with graphene nanopores24,25,26. Alternative means of DNA sequence readout has been experimentally explored, including measurements of the electrochemical current off the edge of graphene27 and graphene nanoribbon conductance28. Experiments using ssDNA have proven to be more difficult29,30,31 and required exquisite control over the properties of the graphene membrane.
Using the all-atom molecular dynamics (MD) method32, we have previously shown that molecules of ssDNA adhere to the surface of graphene and, when driven by an external electric field, translocate through a nanopore in discrete, often single-nucleotide steps16. Our atomic-resolution Brownian dynamics simulations33 predicted a measurable dependence of the nanopore ionic current on the type of DNA nucleotides confined within a graphene nanopore16. Thus, a graphene nanopore system appears to have all the features that have made DNA sequencing using biological nanopores possible34,35. However, just as when a biological enzyme is used to thread a DNA strand through a nanopore, the stepwise motion of ssDNA through a graphene nanopore is stochastic. A deterministic trap-and-release control over DNA transport through a nanopore36 can considerably reduce the stochastic variation in the duration of individual translocation steps and thereby increase fidelity of DNA sequence detection.
Here we report the effect that electric charge on graphene has on the conformation of adsorbed ssDNA and on the velocity of electrophoretic motion of ssDNA through a graphene nanopore. Surprisingly, we find the conformation of ssDNA to depend sensitively on both the sign and magnitude of the graphene charge and on the nucleotide composition of the DNA strand. We also show that the charge of a graphene membrane can be used to regulate the velocity of nanopore transport. Our results open new avenues for using graphene in biosensing, in particular, nanopore sequencing of nucleic acids.
Figure 1a illustrates a typical system considered in this work. A graphene membrane containing a single 1.6-nm-diameter nanopore is submerged in 1 M KCl solution; a DNA molecule is threaded half-way through the nanopore. An external electric field is applied to produce a transmembrane gradient of electrostatic potential Vs (ref. 32). Carbon atoms comprising the graphene membrane are assigned partial charges to electrostatically bias the graphene membrane with respect to solution. Figure 1b illustrates the distribution of the electrostatic potential in a typical simulation system. The electrostatic potential drops monotonically along the nanopore axis but attains elevated values within the graphene membrane (Fig. 1c). An approximate relationship between the membrane charge density σ and the membrane potential Vm can be determined by computing the average change in the electrostatic potential of the graphene membrane upon the assignment of the membrane charge density σ. A more precise characterization of the relationship would require a multi-scale approach23,37 to take the electronic structure of graphene into account. The surface charge densities considered in this work are rather realistic; for example, the charge density of a silica surface can vary between 0 and −5 e nm−2, depending on the fabrication procedures38. Hereafter, e denotes the charge of a proton.
Effect of graphene charge on the conformation of adhered DNA
The charge of a graphene membrane can dramatically alter the conformation of adhered ssDNA. Figure 2 illustrates the results of MD simulations performed in the absence of an external electric field (Vs=0), a poly(dT)20 strand, a two-layer membrane and the surface charge density of 0, −2 and +2 e nm−2. On charge-neutral graphene (σ=0), the bases of ssDNA adhere to the surface of the membrane as the phosphate groups of the DNA backbone remain unbound from the surface (Fig. 2a,b). Changing the membrane charge density from 0 to −2 e nm−2 was observed to unbind ssDNA from graphene (Fig. 2c), indicating the dominance of electrostatic repulsion between the electronegative phosphate groups of DNA and the negatively charged graphene surface over the attractive, hydrophobic ssDNA—graphene forces. The charge-induced unbinding of ssDNA is reversible: upon changing the membrane charge density back to zero, the poly(dT)20 strand was observed to rebind to the graphene surface. Figure 2d and Supplementary Movie 1 illustrate an MD trajectory where the DNA binding–unbinding cycle was repeated six times. Although unbinding of ssDNA was observed to occur almost instantaneously (within 1 ns), rebinding of all the bases onto a charge-neutral membrane required considerably longer time intervals (≈50 ns). The rebinding time scale is determined by the diffusive motion of ssDNA and spontaneous disruption of the nonspecific secondary structure formed by the unbound fragments of ssDNA.
Figure 2e,f illustrates the effect of a positive membrane charge (σ=+2 e nm−2) on the conformation of ssDNA. Although the bases of ssDNA remain bound to the membrane, they are no longer parallel to the membrane and tilt at a ~47° angle. At the same time, the phosphate groups of the backbone attach to the membrane surface (Fig. 2f). The flat (σ=0 e nm−2) and tilted (σ=+2 e nm−2) conformations can be easily distinguished from one another by computing the distances between the graphene membrane and the centres of mass of the base and phosphate groups (Fig. 3a,b). The force-driving ssDNA into the tilted conformation is electrostatic: the negatively charged backbone is attracted towards the positively charged membrane while the base tilts to minimize the electrostatic energy of its dipole moment. The methyl groups’ exposure to water is minimized by the base stacking of the tilted nucleotides (Fig. 2f). The change between flat and tilted conformations is reversible and occurs in less than 10 ns. Figure 2g and Supplementary Movie 2 illustrate an MD trajectory where the flat–tilt cycle was repeated six times. In the tilted conformation, the DNA strand remains mobile on the graphene surface, diffusing around the nanopore.
Figure 3c specifies the range of conformations adopted by a poly(dT)20 strand on a two-layer charged graphene membrane. Regardless of the graphene surface charge density, about 2 out of 20 nucleotides reside within the nanopore, on average. For |σ|≤ 1 e nm−2, the remaining bases are bound flat to the surface of graphene as shown in Fig. 3c. The number of unbound nucleotides increases as σ decreases from −1 to −2 e nm−2. Conversely, for the charge density exceeding +1 e nm−2, the number of nucleotides adopting a tilted conformation increases with the charge density (Fig. 3c). However, even at high-charge densities, a number of nucleotides remain bound flat to the surface. At σ=+2.25 e nm−2, a new population of nucleotide conformation (backbone bound) emerges: the nucleotides can have both their base and backbone bound to the membrane (Fig. 3b).
Conformations of DNA homopolymers on charged graphene
The unique atomic structure of DNA nucleotides makes the conformational response of a DNA strand to a change in the graphene charge dependent on the nucleotide composition of the DNA strand. Specifically, we found homopolymers of thymine, guanine, cytosine, methylated cytosine and adenine nucleotides to adopt statistically different conformations at a graphene surface of the same charge density (Fig. 4). On charge-neutral graphene, DNA nucleotides of all types adopt the same flat conformations, similar to that shown in Fig. 2a,b. A negative charge on a graphene surface repels the negatively charged phosphate group of a DNA backbone. For a poly(dT) strand, this repulsion produces unbinding of the strand from the graphene surface at σ<−1.5 e nm−2. However, several factors can influence the energetics of the surface charge-induced unbinding: the strength of hydrophobic base–graphene interactions, the distribution of partial charges on the nucleotides and the ionic strength of the solution. For example, we found the conformational response of ssDNA to graphene charging in vacuum to be qualitatively different from that in ionic solution, see Supplementary Fig. 1.
Unlike poly(dT), poly(dC) remains partially bound to the graphene surface at σ=−2.0 e nm−2 (Fig. 4a); the unbound cytosine bases form a base-stacking pattern near the pore opening, see Supplementary Movie 3. Compared with poly(dC), a homopolymer of methylated cytosines, poly(dmC), exhibits a more pronounced unbinding, albeit having more bases bound to the surface than poly(dT) (Fig. 4b,c). The nucleotides of poly(dmC) adopt a variety of conformations, including flat, unbound and upright. In the latter case, the nucleotides make contact with the graphene surface through their amine and methyl groups, see Supplementary Movie 4. On the other hand, the majority of nucleotides of a poly(dA) strand unbind from the graphene surface at σ=−2.0 e nm−2 (Fig. 4d). The unbound adenine bases can form a tight base-stacked structure over the opening of the pore, see Supplementary Movie 5. Strikingly, the majority of nucleotides in a poly(dG) strand adopt an upright conformation (Fig. 4e,f), in which the backbone of a DNA strand is detached from graphene, whereas the amine groups of each dG base remain in contact with the graphene surface (see Supplementary Movie 6). The upright conformation of dG is seen only at σ=−2.0 e nm−2; at lower magnitude negative charges, dG bases remain partially adhered to graphene. Because of the relatively short duration of our simulations, the homopolymer of guanines was not expected to form a G-quadruplex39. Figure 4g quantitatively describes the ensemble of conformations sampled by each homopolymer at σ=−2.0 e nm−2. With the exception of poly(dA) and poly(dT), each homopolymer has a unique conformation.
At σ=+2.0 e nm−2, the phosphate groups of the backbone generally adhere to the membrane, whereas the DNA bases either tilt or unbind from the membrane (Fig. 4h–o). There are, however, considerable differences in the behaviour of DNA homopolymers. In a poly(dC) strand, the majority of DNA bases remain bound to the membrane, although a few bases tilt to varying angles, see Supplementary Movie 7. Under the same conditions, a considerable fraction of nucleotides from the poly(dmC) strand have their bases completely detached from the graphene surface (Supplementary Movie 8). In a poly(dA) strand, the majority of nucleotides have their bases detached from the membrane (Supplementary Movie 9). A poly(dG) strand contains a mixture of tilted, unbound and bound flat bases (Supplementary Movie 10). Overall, poly(dA) has the greatest number of unbound bases, followed by poly(dG) and poly(dmC). Figure 4p quantitatively describes the ensemble of conformations observed for each homopolymer at σ=+2.0 e nm−2. On average, each homopolymer adopts a distinct conformation.
To estimate the effect of graphene charging on the conformation of a random-sequence DNA strand, it is useful to note that the interactions of nucleotides with graphene is short-range because of both exponentially decreasing base-stacking40 and screened electrostatic interactions. Neglecting secondary structure formation, the conformational effects should be local, about 1 nm in range, which correspond to the nearest neighbours in the DNA sequence space. The local, additive nature of the interaction can permit, in principle, extraction of some information about the DNA sequence from the conformational transformations upon charging, such as the percentage of nucleotides of certain types, single-nucleotide polymorphism or degree of methylation. In the presence of a competing secondary structure, one can expect to observe a rather complex conformation behaviour. Solvent additives, such as urea, or temperature can be used to suppress secondary structure formation and make the conformational response of a heterogeneous-sequence DNA more predictable.
Graphene charge controls nanopore transport of ssDNA
The charge of a graphene membrane can have a pronounced effect on the electrophoretic velocity of ssDNA in a nanopore (Fig. 5a). The initial conformations for each of the seven simulations shown in the figure were obtained from a ~60 ns equilibration (Vs=0) at the target charge density. A transmembrane bias of ±500 mV was applied in each simulation. For several systems, the sign of the bias was switched during the simulation to prevent ssDNA from escaping the nanopore.
Figure 5b shows the average translocation velocity of poly(dT) as a function of the membrane charge density. For a charge-neutral membrane, the average translocation velocity is 0.06 nt ns−1. The translocation velocity increases with the charge density, reaching 0.25 nt ns−1 at +1 e nm−2. Despite the increase, the DNA translocation may still occasionally halt. The translocation velocity drops by at least two orders of magnitude at σ≤−1.0 e nm−2, regardless of whether the strand remains partially bound to (−1.0 and −1.5 e nm−2) or completely unbound from (−2.0 e nm−2) the graphene surface. Similar dependence of the translocation velocity on the membrane charge density was observed for DNA homopolymers consisting of A, C, mC and G nucleotides (Fig. 5c and Supplementary Fig. 2). The effect appears to depend on the number of carbon layers: the three-layer graphene membrane has shown the most reliable control over DNA transport, whereas transport of ssDNA occurred at all charge densities studied for single-layer graphene (Supplementary Fig. 3).
The highly nonlinear dependence of the DNA translocation velocity on the graphene charge density can be used to control the velocity of DNA transport without altering the transmembrane bias. Figure 6 illustrates a proof-of-principle simulation of such velocity control. At the beginning of the simulation, the three-layer membrane was positively charged (σ=+1.5 e nm−2), which was expected to promote translocation of ssDNA. Indeed, under a +500-mV bias, ssDNA was observed to move by ~7 nucleotides through the nanopore until the membrane charge density was switched to −1.5 e nm−2, which arrested the translocation. Then, the sign of the transmembrane bias was reversed; the transport of ssDNA remained arrested by the negative charge density of the membrane. The charge of the membrane was then switched to +1.5 e nm−2, which restarted the translocation of ssDNA, and then back to −1.5 e nm−2, which arrested the transport. The sign of the transmembrane bias was reversed; the stop-and-go motion of ssDNA was produced again by switching the membrane’s charge density. In total, two cycles of stop-and-go motion of ssDNA strand were demonstrated within this 860 ns simulation. Supplementary Movie 11 illustrates this simulation trajectory. Similar control over DNA motion was observed in our simulations of a two-layer membrane system (Supplementary Fig. 4). In the latter case, the stop-and-go motion was less robust than in the three-layer system, which may be explained by weaker interactions of DNA with the nanopore. The presence of DNA in the nanopore introduced minor perturbations in the distribution of the electrostatic potential along the nanopore axis (Supplementary Fig. 5).
For a random-sequence DNA strand, one can expect to observe the same kind of stop-and-go motion as along as its secondary structure does not prevent its bases from adhering to the graphene membrane. For a DNA strand adhered to a graphene membrane, the interaction of the strand with the graphene membrane can be approximated as a superposition of interactions between individual nucleotides and the membrane. Hence, the transport rate modulations observed for the DNA homopolymers (Fig. 5b,c) will determine the transport rate of a random-sequence DNA polymer. The possibility of transporting ssDNA in on-demand discrete steps may facilitate DNA sequencing if combined with a rapid DNA sequence detection method, such as a transverse conductance measurement17,18,19,20,21,22,23.
In this study, we have considered an idealized system of a single nanopore made in a defect-free graphene sheet having a uniform distribution of the charge along its surface. However, the charge distribution near the edge of graphene can differ from that in the rest of the membrane. To prove that our results still hold even if the charge distribution is altered, we simulated a system that had the annulus of the pore neutralized. The results of these simulations are shown in Supplementary Figs 6 and 7. Even in such a partially neutralized system, the structural response of DNA to charging of the graphene and the modulations of the DNA translocation velocity were found to be very similar in magnitude to those observed in the case of a uniformly charged graphene membrane. It may also be possible that adsorption of ssDNA to graphene can alter the charge distribution within graphene. However, our analysis of the charge distribution in MD simulations performed with and without a DNA strand (Supplementary Fig. 8) indicates that the effect of the DNA charge on the membrane potential is rather minor (<25 mV) and can be neglected for the graphene charge densities considered.
In summary, electrical charging of a graphene membrane was found to alter the conformations of ssDNA homopolymers bound to the membrane in a manner dependent on the nucleotide composition of ssDNA. The charge of a graphene membrane was shown to regulate the velocity of nanopore transport of ssDNA. Furthermore, switching of the graphene charge density under constant transmembrane bias was shown to produce stop-and-go motion of ssDNA, which is highly desirable for DNA sequencing applications. The described effects can be observed for other atomically smooth hydrophobic surfaces that can alter their electric charge density and biopolymers containing charged and hydrophobic groups, such as unfolded proteins. Although electrochemical effects can limit applicability of certain materials for practical realization of the conformation and transport control principle, properties of the solution can be adjusted to fine-tune the balance of hydrophobic and electrostatic forces and thereby achieve the desirable control over the conformation and transport of biopolymers.
General MD protocols
All MD simulations were performed using the programme NAMD41, a 2-fs integration time step, 2-2-6 multiple time-stepping, CHARMM27 parameter set, a 7- to 8-Å cutoff for van der Walls and short-range electrostatic forces, the particle mesh Ewald (PME) method for long-range electrostatics42 computed over a 1.1-Å grid and periodic boundary conditions. Simulations in the NPT ensemble (constant number of particles N, pressure P and temperature T) were performed using a Lowe-Andersen thermostat43, and Nosé-Hoover langeivn piston pressure control44 set at 295 K and 1 atm, respectively. Visualization and analysis were performed using the Visual Molecular Dynamics (VMD) programme.45.
MD simulations of graphene-DNA systems
All models of graphene sheets were generated using the Inorganic Builder plugin46 of VMD45. Carbon atoms in the graphene sheets were modelled as type CA atoms of the CHARMM force field16,47. Nanopores in graphene were generated by removing carbon atoms whose x, y coordinates fulfilled the condition x2+y2<r2, where r was the radius of the pore. After removing the initial selection of carbon atoms, singly bonded carbon atoms at the edge of the pore were removed as well. The graphene membranes considered in this study contained one, two or three carbon layers and had a single circular pore 8 Å in radius. It was previously determined that nanopores of such dimensions facilitate stepwise translocation of ssDNA16.
To model the effect of electrical bias applied to the graphene membrane, atoms of graphene were assigned partial charges to produce charge densities ranging from −2 to +2 e nm−2. The distribution of partial charges among carbon atoms comprising the membranes depended on the number of carbon layers. For a single sheet of carbons, the charge was evenly distributed among all carbon atoms. For two-layer systems, each carbon atom was assigned the same partial charge to produce the charge density in each of the two carbon layers equal to the half of the target charge density. For example, to model a two-layer graphene membrane charged at 1.0 e nm−2, carbon atoms in each layer were assigned a partial charge of 0.0144, e so that the charge density in each layer was 0.5 e nm−2. For a three-layer membrane, the charge was assigned only to the top and bottom layers of the membrane; the interior layer remained uncharged.
Each graphene nanopore system was combined with an all-atom model of ssDNA that was threaded halfway through the membrane. The initial conformation of ssDNA was that of a single strand of B-form duplex DNA. Each system was solvated using the Solvate Plugin of VMD. Potassium and chloride atoms were added to produce an electrically neutral 1.0 M solution of KCl using the Autoionize Plugin of VMD.
With the exception of the poly(dT) systems, each final system was a hexagonal prism 71.3 Å on side and 91.4 Å in height and contained approximately 43,200 atoms; hexagonal periodic boundary conditions were applied in the xy-plane. The system used for simulations of poly(dT) was smaller and measured 46.7 Å on the side and 92.1 Å in the direction normal to the membrane. The systems were minimized for 480 steps using the conjugate gradient method, followed by a 50- to 60-ns equilibration in the NPT ensemble during which only the dimension normal to the membrane was allowed to change.
All production simulations of ssDNA translocation were performed in the NVT ensemble (constant number of particles N, volume V and temperature T) by applying an external electric field in the z direction. The electric fields are reported in terms of a transmembrane voltage difference Vs=−ELz, where E is the strength of the electric field and Lz is the length of the simulation system in the z direction. Taking the periodic boundary conditions into account, our simulations correspond to an infinite system of graphene membrane/electrolyte layers having a prescribed drop of the electrostatic potential per layer. To prevent membrane layers from drifting, harmonic restraints were applied to the outermost carbon atoms at the edges of the period cell; the spring constant of each harmonic restraint was 40 kcal mol−1 Å−2.
To adjust the charge of a graphene membrane during a stop-and-go simulation, the course of the simulation was interrupted; the latest atomic coordinates were used to build a new system featuring identical conformations of DNA and graphene but modified partial charges of the carbon atoms in the graphene membrane. To preserve the overall electrical neutrality of the system, a small number of potassium ions (less than 10% of the total number of ions) were randomly added to or removed from the system. A short (500 ps) NPT equilibration immediately followed each charge modification. The conformation of ssDNA was harmonically restrained during the 500-ps equilibration, whereas ions and water rearranged, screening the charge of the DNA and membrane. After the 500-ps equilibration, constraints were removed from ssDNA and the production simulation in the NVT ensemble continued.
Calculation of the electrostatic potential
To visualize the distributions of the electrostatic potential in our systems, we averaged the instantaneous distributions of the electrostatic potential over the MD trajectory using the PMEpot Plugin48 of VMD. Each atom of the system was approximated by a spherical Gaussian
where β was the Gaussians’ width. The instantaneous distribution of the electrostatic potential corresponding to the instantaneous charge configuration was obtained by solving the Poisson equation
To obtain the average distribution of the potential in a given MD simulation, instantaneous distributions of the potential were averaged over the entire MD trajectory. The electrostatic potential maps were obtained by averaging 20–30 ns fragments of MD trajectories; β=0.1 A−1 was used for these calculations. One-dimensional profiles of the electrostatic potential across the graphene membranes or through the nanopores were obtained by averaging the corresponding regions of the three-dimensional electrostatic potential maps.
Classification of DNA conformations
The conformations of DNA nucleotides were characterized by computing the nearest distances from the nucleotides’ base and phosphate groups to the graphene surface, as illustrated in Fig. 3a. For a given MD trajectory, normalized two-dimensional histograms were constructed using the graphene-base and graphene-phosphate distances as independent parameters. An example of such a histogram is shown in Fig. 3b. The histograms were constructed for all nucleotides in a DNA strand using the last 40 ns of a 60 ns equilibration trajectory. Typically, the histograms contained several distinct peaks that could be associated with the particular conformations of individual nucleotides with the exception of nucleotides not bound to the surface, which were characterized as such using a distance cutoff of ~7.6 Å for the base and ~5.6 Å for the backbone. To quantitatively characterize the number of nucleotides bound in a particular conformation, a least-squares fit was used to approximate the normalized histograms as a set of Gaussian distributions. The average number of nucleotides adopting a particular conformation was determined by computing the volume of the respective distribution.
The triangle diagrams (Fig. 4g,p) were created by averaging the number of nucleotides categorized to adopt one of the three specific conformations during the last 40 ns of the corresponding equilibration trajectories. The average numbers were normalized by the total number of nucleotides located outside of the nanopore (17.5 on average) and multiplied by 100 to obtain the percentage makeup. The conformations were categorized as follows. In the flat conformation, the base of a nucleotide is bound to the membrane (as defined above). Figure 2b shows an example of such conformation. For the negative charge density, unbound describes a conformation where both backbone and base are detached, as depicted in Fig. 2c. For the positive charge density, the unbound conformation describes nucleotides that have their backbones bound to and bases detached from the graphene membrane (Fig. 4n). In the tilted conformation, the centre of mass of the base is located 5.6–7.4 Å away from the graphene (Fig. 2f). In the upright conformation, the backbone of a DNA nucleotide is detached from graphene while the amine groups of the base are in contact with graphene (Fig. 4e).
Calculation of the number of permeated nucleotides
To quantitatively characterize the process of DNA translocation, the number of DNA atoms located at the trans, grounded side of the membrane was counted for each microscopic state recorded in the MD trajectory. That number was divided by the number of atoms in one nucleotide to give the number of nucleotides located at the trans side of the membrane. A change in that number reported on the progress of DNA translocation.
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This work was supported by the grants from the National Science Foundation (DMR-0955959), the National Institutes of Health (R01-HG007406) and through a cooperative research agreement with the Oxford Nanopore Technologies. We gladly acknowledge supercomputer time provided through XSEDE Allocation Grant MCA05S028 and the Blue Waters petascale supercomputer system (UIUC).
The authors declare no competing financial interests.
Supplementary Figures 1-8 (PDF 2224 kb)
The conformational dynamics of a poly(dT)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene alternates between 0.0 (yellow) and -2.0 (green) e nm-2. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 750 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. Figure 2d quantitatively characterizes the changes in the conformation of DNA. (MOV 10822 kb)
The conformational dynamics of a poly(dT)20 strand threaded through a nanopore in a two-layer grapheme membrane. The charge density of graphene alternates between 0.0 (yellow) and +2.0 (red) e nm-2 . The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 394 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. Figure 2g quantitatively characterizes the changes in the conformation of DNA. (MOV 11901 kb)
The conformational dynamics of a poly(dC)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is -2.0 e nm-2; the left and right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 59 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. The cytosine bases closest to the pore unbind from the surface and form a base stacking pattern whereas bases furthest away remain partially bound to the surface (MOV 11978 kb)
4. The conformational dynamics of a poly(dmC)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is -2.0 e nm-2; the left and right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 79 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. The methylated-cytosine bases either completely unbind from the surface, partially unbind or remain completely bound to the surface. (MOV 14190 kb)
The conformational dynamics of a poly(dA)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is -2.0 e nm-2; the left and right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 99 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. The adenine bases unbind from the graphene surface and form a base-stacked structure over the opening of the pore. (MOV 5394 kb)
The conformational dynamics of a poly(dG)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is -2.0 e nm-2; the leftand right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 81 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. The guanine nucleotides partially unbind from the graphene membrane adopting an "upright" conformation (MOV 6069 kb)
The conformational dynamics of a poly(dC)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is +2.0 e nm-2; the left and right panels show the view of the same systems from the cis and trans sides of the membrane,respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 60 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. Most cytosine bases remain bound to the membrane, however a few tilt at various angles. (MOV 10473 kb)
The conformational dynamics of a poly(dmC)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is +2.0 e nm-2; the left and right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 79 ns of a continuous MD trajectory. The transmembrane bias is zero throughout the simulation. The methylated-cytosine bases either tilt at various angles or unbind from the membrane. (MOV 7390 kb)
The conformational dynamics of a poly(dA)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is +2.0 e nm-2; the leftand right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue(nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 100 ns of acontinuous MD trajectory. The transmembrane bias is zero throughout the simulation. The majority of the adenine bases completely unbind from the membrane. (MOV 5736 kb)
The conformational dynamics of a poly(dG)20 strand threaded through a nanopore in a two-layer graphene membrane. The charge density of graphene is +2.0 e nm-2; the leftand right panels show the view of the same systems from the cis and trans sides of the membrane, respectively. The DNA is shown using vdW spheres colored according to the atom type: blue (nitrogen), red (oxygen), white (hydrogen) and gold (phosphorous). The movie covers 81 ns of acontinuous MD trajectory. The transmembrane bias is zero throughout the simulation. The guanine bases adopt unbound, tilted or completely bound conformations. (MOV 7349 kb)
Stop-and-go transport of a poly(dT)20 strand through a nanopore in a 3- layer graphene membrane. The presence of a ±500 mV transmembrane bias is schematically indicated by the color of the background. The motion of ssDNA strand is controlled by the charge of the graphene membrane: the motion is facilitated at +1.5 e nm-2 (red) and arrested at -1.5 e nm-2(green). The DNA is shown using vdW spheres colored according to the nucleotide number. The movie illustrates 860 ns of a continuous MD trajectory. The MD trajectory is paused during each membrane charge adjustment; the actual time scale of the charge adjustment is 0.2 ns. (MOV 9304 kb)
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Shankla, M., Aksimentiev, A. Conformational transitions and stop-and-go nanopore transport of single-stranded DNA on charged graphene. Nat Commun 5, 5171 (2014). https://doi.org/10.1038/ncomms6171
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