(a) AUC statistics are shown for the ERCC controls at the three differential ratios. (b) LODR count estimates (on a log scale) for the ERCC controls at the three differential ratios. Error bars represent LODR bootstrap method 90% confidence intervals. (c) Weighted mean estimates of mRNA fraction differences for the sample set with error bars representing weighted standard errors. The solid black line represents the measurement of rm from previous work22 and dashed black lines show the confidence interval from the standard deviation of this estimate. (d) Violin plots show distributions of ERCC control ratio standard deviations at each laboratory. The legend is common to all figures, colour indicates measurement process and transparency of each colour is used to indicate results for different ratios.