Figure 6 : Feeding behaviour microstructure reveals homeostatic strategies.

From: Automated monitoring and quantitative analysis of feeding behaviour in Drosophila

Figure 6

(a) Illustration of the temporal dynamics of feeding of a fly on gelatinous food and of the different parameters measured and analyzed using the flyPAD approach. (b) Mean number of sips per burst, (c) mean IBI length and (d) mean duration of activity bouts in fully fed, 4 h starved and 8 h starved animals. (e) Cumulative feeding for the same three groups. Line represents the mean and the shading the s.e.m. (f) Number of sips per burst, (g) duration of the IBI and (h) duration of activity bouts for five consecutive 10-min time windows over the course of a meal. In fh, lines represent the mean and the shading the s.e.m.; significance was tested compared with the first window. (i) Linear and (j) quadratic coefficients extracted from quadratic fit to the cumulative feeding of individual flies (Supplementary Fig. 1) in fully fed, 4- and 8-h starved conditions. (k) Model of how hunger and satiation induce stepwise changes in feeding strategies to achieve homeostasis. Box plots display the median, interquartile range and 5–95 percentile whiskers, with data beyond these whiskers shown as points. NS, not significant (P>0.5), *P≤0.05, **P≤0.01, ***P≤0.001, Significance was tested by Wilcoxon Rank-Sum test with Bonferroni correction, n=24.