Figure 7 : Mobility of GFP3 in the nucleus after decondensation of chromatin.

From: Retrieving the intracellular topology from multi-scale protein mobility mapping in living cells

Figure 7

(a) Time-dependent diffusion coefficient of inert GFP3 in the nucleus of U2OS cells after perturbation of the chromatin structure with TSA (blue, n=15 cells) compared with that in unperturbed cells (black, n=16 cells). (b) Same as in a but after perturbation with CQ (blue, n=13 cells). (c) Mobility of STAT2 (red, n=12 cells), GFP3 (white, n=15 cells) and GFP5 (black, n=18 cells) in the cytoplasm of U2OS cells. The STFM image shows a representative cell expressing GFP-tagged STAT2. (d) Mobility of the chromodomain (CD) of HP1β in the cytoplasm (red, n=11 cells) and nucleus (blue, n=11 cells) compared with that of cytoplasmic GFP1 (black, n=13 cells). The STFM image shows a representative cell expressing GFP-tagged CD. Data are mean values±s.e.m., fit curves correspond to Equation (1). Scale bar, 5 μm.