Table 2 Importance of efficacy criteria on in vivo gene silencing.

From: Degradable lipid nanoparticles with predictable in vivo siRNA delivery activity

No of Criteria met Tertiary amine O13 tail > 2 tails pKa>5.4 >95% silencing (% of formulations)
4 X X X X 100
3 X X X   0
  X   X X 56
  X X   X 17
   X X X 67
2 X X    0
  X   X   0
  X    X 0
   X X   0
   X   X 0
    X X 0
1 X     0
   X    0
    X   0
     X 0