Figure 5 : Sestrin2 prevents liver damage during obesity.

From: Hepatoprotective role of Sestrin2 against chronic ER stress

Figure 5

(ac) Livers and sera were collected from 6-month-old WT (n=6) and Sesn2−/− (n=5) mice kept on HFD for 4 months (HFD panels) or from 4-month-old Lepob/ob/Sesn2+/− (Con, n=4) and Lepob/ob/Sesn2−/− (n=6) mice kept on LFD (Lepob panels). Liver sections were subjected to TUNEL (red) and DAPI (blue) staining (a). TUNEL-positive cells were quantified (b). Serum ALT levels were quantified from indicated groups of mice (c). (df) Livers from obese Sesn2−/− mice transduced with AAV-Con (n=4) or AAV-Sesn2 (n=3) were subjected to TUNEL (red) and DAPI (blue) staining (d). TUNEL-positive cells in livers (e) and serum ALT levels (f) were quantified. (gi) Five-month-old Sesn2−/− mice kept on HFD for 3 months were daily injected with vehicle (PBS, n=4) or TUDCA (500 mg per kg body weight per day i.p., n=5). After 10 days of the treatment, mice were killed, and livers and sera were collected. Livers were subjected to TUNEL (red) and DAPI (blue) staining (g). TUNEL-positive cells in livers (h) and serum ALT levels (i) were quantified. Scale bars, 100 μm. All data are shown as the mean±s.e.m. P values are from Student’s t-test.