Figure 3 : Sestrin2 deficiency exacerbates ER stress upon chemical insults or obesity.

From: Hepatoprotective role of Sestrin2 against chronic ER stress

Figure 3

(a,b) Two-month-old WT or Sesn2−/− mice kept on LFD were injected with Tm (500 mg per kg body weight, i.p.). After indicated hours, livers were harvested from the treated mice and analysed (n=4). Protein phosphorylation and expression were analysed by immunoblotting (images with black bands) (a) and quantified (b). XBP1 mRNA splicing was examined through semi-quantitative RT–PCR (images with white bands) (a). (ci) Six-month-old WT (n=6) and Sesn2−/− (n=5) mice kept on LFD or HFD for 4 months (cf) and 4-month-old Lepob/ob/Sesn2+/− (Con, n=4) and Lepob/ob/Sesn2−/− (n=6) mice kept on LFD (gi) were analysed. Protein phosphorylation and expression were analysed by immunoblotting (c,g) and quantified by densitometry (d,h). Liver sections were stained with indicated antibodies (e). Relative mRNA expression of ER stress-inducible genes was quantified through qRT–PCR (f,i). Scale bars, 100 μm. All data are shown as the mean±s.e.m. P values are from Student’s t-test. Molecular weight markers are indicated in kDa (immunoblots) or bp (agarose gels).