Figure 1 : Obesity induces Sestrin2 through ER stress signalling.

From: Hepatoprotective role of Sestrin2 against chronic ER stress

Figure 1

(ac) HepG2 cells were treated with PA for indicated hours and analysed by immunoblotting (images with black bands) and RT–PCR (images with white bands). P values were calculated between untreated (0 h) and indicated groups (b,c; n=3). (d,e) At 48 h after infection with green fluorescent protein (GFP; Con)- or SERCA2b-overexpressing adenoviruses, cells were treated with BSA (0 h) or PA for indicated hours (d) or 9 h (e; n=3). (f,g,l,m) Six-month-old WT mice kept on low-fat diet (LFD) or HFD for 4 months were injected daily with vehicle (phosphate-buffered saline (PBS)) or TUDCA (500 mg per kg body weight i.p.) or transduced once with adenoviruses expressing SERCA2b, GFP (Con) or shRNA targeting c/EBPβ. After 10 days, livers were analysed by immunoblotting (n=3). (h,k) At 48 h before PA treatment, cells were infected with lentiviruses expressing shRNAs targeting luciferase (Con), PERK (h) and c/EBPβ (k). After 9 h of BSA (−) or PA treatment, cells were analysed by immunoblotting. (i,j) ChIP analysis of cells with indicated treatments (n=3). (n) At 24 h after infection with GFP (Con)- or c/EBPβ-overexpressing adenoviruses, cells were analysed by immunoblotting. All data are shown as the mean±s.e.m. P values are from Student’s t-test. Molecular weight markers are indicated in kDa (immunoblots) or bp (agarose gels).