The greatest potential environmental benefit of metabolic engineering would be the production of high-volume commodity chemicals, such as biofuels. Yet, the high yields required for the economic viability of low-value chemicals is particularly hard to achieve in microbes owing to the myriad competing biochemical pathways. An alternative approach, which we call synthetic biochemistry, is to eliminate the organism by constructing biochemical pathways in vitro. Viable synthetic biochemistry, however, will require simple methods to replace the cellular circuitry that maintains cofactor balance. Here we design a simple purge valve module for maintaining NADP+/NADPH balance. We test the purge valve in the production of polyhydroxybutyryl bioplastic and isoprene—pathways where cofactor generation and utilization are unbalanced. We find that the regulatory system is highly robust to variations in cofactor levels and readily transportable. The molecular purge valve provides a step towards developing continuously operating, sustainable synthetic biochemistry systems.
Metabolic engineering and synthetic biology have been employed for the production of high-value chemicals but has not been as successful in meeting the stringent economics of large-scale commodity chemical manufacturing1. Microbial systems are often hampered by a variety of technical challenges that make it hard to achieve cost competitiveness, including poor yields owing to competing pathways; low productivity caused by slow growth rates or difficulties in pathway optimization; contaminating microbial growth; product toxicity; and expensive product isolation2,3.
Another approach that is beginning to receive attention is to perform complex biochemical transformations using mixtures of enzymes in a reaction vessel rather than within a cell. Building single, dedicated pathways in vitro can eliminate side reactions that occur in the cell, so that nearly 100% yields and fast reaction times are possible3,4,5. In vitro biochemical systems also allow for more precise control over optimization and product toxicity problems can be more easily diagnosed and mitigated6,7. Moreover, product extraction can be more facile.
Traditionally, in vitro pathway construction has been relegated to use as a research tool or in applications that require only 1—3 enzymes for the production of chiral compounds and other high-value chemicals1,8,9. Improvements in protein expression and access to stable enzymes have made more complex systems possible, however1,9,10,11,12. In vitro biotransformation systems have been reported in recent years involving systems of over 30 enzymes13,14. One of the first modern studies in this area was an artificial pathway that produced hydrogen from starch1,15,16,17. The concept was recently advanced with a creative system that generated hydrogen from cellobiose at nearly 100% yields16. In another effort, hyper-thermophilic glycolysis enzymes were heterologously expressed, heat purified and assembled to convert glucose to n-butanol in 82% yield18. In another study, an elegantly simplified non-phosphorylative Entner–Doudoroff pathway from hyper-thermophilic archaea was constructed to produce ethanol and isobutanol in ~55% yields19. These pioneering studies illustrate the flexibility of synthetic biochemistry and the potential for high yields.
A limiting feature of earlier work in synthetic biochemistry systems is the need to either feed the systems with high-energy cofactors, or if they are generated by the pathway in situ, balance their use to ensure regeneration. For example, in the Sieber pathway to generate ethanol and isopropanol from glucose19, the pathway was beautifully designed to reduce only 2 mol of NAD+ to NADH during the catabolic phase and to reoxidize 2 mol of NADH in the anabolic phase. Thus, the utilization of NAD+ was perfectly balanced allowing multiple passes through the pathway. In other systems, the pathways were also designed to maintain cofactor balance18.
The requirement for perfect balance limits synthetic biochemistry in two ways, however. First, it places stringent constraints on pathway design, potentially limiting the types of chemicals that can be made. Second, it may ultimately limit the number of cycles the system can perform without adding additional, expensive high-energy cofactors. Why? If there is any spontaneous oxidation of NADH, it effectively creates an unintended cofactor imbalance because more NADH is required than would be expected based on the stoichiometry of the system. Thus, over time the levels of NADH will dissipate and the system will wind down. As the economic viability of a synthetic biochemistry system will likely depend on the ability to run the systems in a self-sustaining manner for long periods of time, we need to develop methods that can maintain high-energy cofactor levels without requiring perfect pathway stoichiometry.
Ideally, we believe that a synthetic biochemistry system should be able to generate more high-energy cofactors in the catabolic phase of the pathway, than is needed in the anabolic phase of the pathway so that gradual losses by spontaneous ATP hydrolysis or NAD(P)H oxidation can be tolerated. On the other hand, if an excess of high-energy cofactors is produced, it is necessary to also find a way to dissipate excess production so that the reactions can proceed (that is, carbon flux can be maintained). In short, we need a way to maintain cofactor balance. If it were possible to regulate cofactor balance without affecting carbon flux, it would also allow for much more flexibility in pathway design. As a step towards this goal, we describe a synthetic biochemistry purge valve module to maintain the proper balance of NADPH and test whether this general approach can be applied for the production of the bioplastic polyhydroxybutyrate (PHB) and isoprene.
PHB and other polyhydroxyalkanoates (PHAs) are biodegradable thermoplastics. PHAs can have characteristics similar to many popular petrochemical-derived polymers, but are nontoxic and biodegradable, so they are attracting increased attention as a possible green alternative to petroleum-based polymers in a wide range of applications20,21,22. The best characterized and most abundant PHA polymer is PHB that is naturally produced from acetyl-CoA as a carbon and energy storage mechanism in many organisms22. Currently, industrial production of PHB is done by in vivo batch culture processes under nutrient starvation. This process is typically very time consuming, requires large fermentation volumes and requires expensive methods for the extraction of PHB20. Prior attempts to produce bioplastic in vitro have required the addition of sacrificial substrates and a molar excess of cofactors to convert acetate to PHB23.
Isoprene is a platform chemical for a variety of products, but it is mostly employed in the production of synthetic rubber24,25,26. The isoprenoid pathway also provides precursors for over 25,000 known biomolecules including drugs such as taxol and potential biofuels27. There have been a number of efforts to produce isoprene in microorganisms24,26,28,29 and the best reported yield is 28% from glucose24. We recently showed that a synthetic biochemistry system could produce isoprene in >95% yield from pyruvate as long as high-energy cofactors were added30.
Here we employ a purge valve system in a pathway to convert pyruvate into PHB that maintains sustainable reducing cofactor balance, without the requirement for perfect stoichiometric matching of cofactor generation and carbon usage. Further, we show that our purge valve module can be used as the basis for the production of other acetyl-CoA-derived products by applying it to the production of isoprene from pyruvate via the mevalonate pathway. Regulatory modules such as this can free us from having to perfectly balance cofactor utilization when designing synthetic biochemistry systems.
Overview and design of pathway
The biotransformation of pyruvate into PHB illustrates a basic cofactor imbalance problem that is encountered in biochemical systems. In particular, conversion of pyruvate to acetyl-CoA by pyruvate dehydrogenase (PDH) yields one molecule of NADH. However, the three-enzyme pathway (phaA, B and C) to PHB from acetyl-CoA utilizes only one half of a molecule of NADPH per acetyl-CoA. Thus, the canonical pathway produces an excess of reducing equivalents. Moreover, the reducing equivalents are of the wrong type (NADH rather than NADPH). We therefore designed a pathway, shown in Fig. 1a that can generate the correct cofactor and regulate its production.
In our design, we create a synthetic biochemistry ‘purge valve’ that effectively decouples the stoichometric production of NAD(P)H from acetyl-CoA (Fig. 1). To this end, we employ a mixture of both an NAD+-utilizing wild-type (WT) PDH (PDHNADH), a mutant PDH that utilizes NADP+ (PDHNADPH), and a water-generating NADH oxidase (NoxE) that specifically oxidizes NADH, but not NADPH31,32. By employing this metabolic node, we generate NADPH needed for PHB production from pyruvate, but also dissipate excess reducing equivalents in an autoregulatory manner. As illustrated in Fig. 1b, under low-NADPH, high-NADP+ conditions, the mutant PDHNADPH can operate to generate acetyl-CoA and restore NADPH levels. Under high-NADPH, low-NADP+ conditions, the PDHNADPH activity will automatically be choked off, and the WT PDHNADH will be used preferentially to produce acetyl-CoA and NADH. In this high-NADPH condition, the reducing equivalents are not needed. Because the reducing equivalents are produced in the form of NADH and not NADPH, they are eliminated by the oxidase, NoxE. The presence of NoxE ensures that NADH never builds up and the PDHNADH can always operate to generate carbon for the PHB pathway in the form of acetyl-CoA. The PDHNADH/PDHNADPH/NoxE system acts like a purge valve that opens under conditions of high NADPH to relieve the excess reducing equivalent ‘pressure’ (that is, buildup of NADH) and allow carbon flux to be maintained. An engineering schematic of the purge valve system is shown in Fig. 1c.
Enzyme engineering and cofactor specificity
To implement our purge valve module, we needed an NADP+-utilizing PDH. A mutant of Escherichia coli PDH has been engineered to have NADP+ specificity by introducing mutations into the E3 enzyme (EcE3)33. The E. coli PDH proved too unstable for our system, however. We therefore engineered a mutant of the thermophilic Geobacillus stearothermophilus PDH that preferentially accepts NADP+ with increased enzyme stability.
Similar to the design of the E. coli PDH mutant, the G. stearothermophilus PDH mutant was designed by overlaying the known structure of the G. stearothermophilus E3 subunit (GsE3)33 with the known structure of the related E. coli glutathione reductase, which utilizes NADP+ (ref. 33) (REF or PDBID:1GET). The structural superposition allowed us to position the additional phosphate moiety in the active site of the GsE3, based on how it was placed in glutathione reductase (see Fig. 2). We could then design side-chain substitutions in GsE3 that might allow acceptance of the phosphate. We were guided by the prior successful design of the EcE3 enzyme, which shares 47% sequence identity with the GsE3 (refs 33, 34). The mutations introduced into EcE3 were E206V, G207R, A208K, G209H and S213R (GsE3 numbering). After examining the changes in the context of the GsE3 structure, we decided to introduce all but G209H, because it appeared that the new His side chain might create steric clashes with nearby K224 and N237 residues.
The kinetic properties of the engineered and WT enzymes reveal that the mutations alter specificity as desired. The kinetic parameters are listed in Supplementary Table 1. For the WT G. stearothermophilus enzyme (GsPDHNADH), kcat is 11.2 times higher with NAD+ than NADP+ and kcat/Km is 1,150 times higher. For the engineered mutant (GsPDHNADPH), kcat is 7.3 times higher with NADP+ than NAD+ and kcat/Km is 21 times higher. Thus, we were able to flip the specificity of the PDH enzyme.
The GsPDHNADH and GsPDHNADPH enzymes (henceforth designated PDHNADH and PDHNADPH) were much more stable than their E. coli counterparts. As shown in Supplementary Fig. 1, the G stearothermophilus enzymes retained ~50% activity after 1 h incubation at 67 °C, whereas the E. coli PDH enzymes were completely inactivated at 50 °C.
A second key requirement of the purge valve design is the use of an NADH oxidase with high cofactor specificity. We chose NoxE from Lactococcus lactis as it is a water-forming NADH oxidase so it does not generate any toxic products such as hydrogen peroxide31,32,35. As shown in Table 1, the Kcat of NoxE is 248.8 times greater with NADH than NADPH and kcat/Km is 9,900 times greater.
Enzyme catalysed production of PHB from pyruvate
The enzymes chosen for use in this work are listed in Table 1. In the initial tests, we only employed the WT, PDHNADH complex and NoxE to generate acetyl-CoA and supplied NADPH exogenously. After optimizing enzyme ratios in this simple system, we then added the mutant PDHNADPH to test in situ generation of NADPH. Finally, the amount of PDHNADPH was optimized, keeping the other enzymes fixed.
The progress of the optimized pyruvate to PHB reaction is shown in Fig. 3 along with a control lacking the last enzyme, phaC. Both reactions had a PDHNADPH:PDHNADH ratio of 40:1. At this ratio, the NADPH levels rise rapidly (A340) and are maintained throughout the time course (NoxE rapidly oxidizes NADH so changes in A340 reflect only changes in NADPH levels). At the same time, PHB granules are produced as monitored by A600 (ref. 36).
We assayed the PHB production using a gas chromatography method and found that the optimized reaction produced 2.45±0.5 mg ml−1 of PHB from 50 mM pyruvate, which represents nearly complete conversion (94±20%) of pyruvate to plastic. In the optimized system, we started with 0.5 mM NADP+, so achieving 94% yield requires over 90 turnovers of the NADP+ cofactor, indicating a high level of system sustainability.
The stability of the full system was assessed by mixing components together and then initiating the reaction at various time delays. The decrease in extent of the reaction is shown in Supplementary Fig. 2. We find that the extent of reaction remains ~50% after 2 days.
Autoregulatory behaviour of purge valve
The regulatory behaviour of the purge valve is better seen at sub-optimal enzyme concentrations and ratios of PDHNADPH to PDHNADH that slow down the response time. In the optimized assay (40:1 mol ratio of PDHNADPH:PDHNADH), we observed a rapid rise in NADPH levels, which was sustained throughout. In the non-optimal systems shown in Fig. 4, the purge valve cannot respond as rapidly to drops in NADPH concentrations so we can observe variations in NADPH levels as the system develops. We still observe a rapid initial rise in NADPH levels, but as intermediates build up, the consumption starts to outstrip NADPH production. Eventually, the system compensates by generating higher levels of NADPH. The restoration of NADPH levels would be impossible without the proper operation of the purge valve system.
The system is robust to cofactor levels
To test whether the system was robust to changes in cofactor levels, we varied the initial cofactor concentrations in the reactions and measured the yields of PHB. Each reaction was constructed with combinations of NAD+, NADH, NADP+ or NADPH at either 0.1 or 1 mM and the production of PHB was monitored by the final OD at 600 nm. All of the reaction conditions were compared with the optimized reaction that produced nearly complete conversion of pyruvate to PHB and, as shown in Fig. 5, were within random variation (see also Supplementary Table 3). This result indicates that the purge valve can compensate readily for changes in cofactor concentrations and reduction states.
Porting the purge valve system to isoprene production
To test the versatility of the molecular purge valve and whether it can be applied as a general platform for the production of a diverse array of acetyl-CoA-derived compounds, we used the PDH purge valve to produce isoprene via the acetyl-CoA-dependent mevalonate pathway. We have previously described the in vitro production of isoprene from pyruvate, which required the use of exogenously added NADPH30. Similar to the PHB pathway, the mevalonate pathway has an inherently different carbon and cofactor stoichiometry. In particular, the mevalonate pathway requires three acetyl-CoA and two NADPH for the production of isoprene (see Fig. 6a). Thus, system sustainability requires generation and regulation of NADPH levels.
We tested whether the purge valve system can replace exogenously added NADPH in the production of isoprene. As shown in Fig. 6b, the full purge valve system produces an 88.2±8.4% yield from 3 mM pyruvate. This yield is even higher than the 81.4±2.0% yield obtained if we add NADPH exogenously (Fig. 6b). If we leave out any of the purge valve components (PDHNADPH, PDHNADH or NoxE), yields are markedly reduced. Thus, the purge valve system is clearly transportable to other synthetic biochemistry systems.
Maintaining proper cofactor balance is an essential part of generating flux and providing a driving force through an enzymatic pathway. In vivo, the enzymatic specificity for the cofactors NADH and NADPH are typically used to control the carbon flux through catabolic and anabolic pathways, respectively. Organisms typically sense the reduction state of these cofactors and use this information to upregulate or downregulate catabolic and anabolic pathways to cope with environmental changes. In vitro systems, however, do not have the myriad peripheral pathways that facilitate this fine control. Moreover, the natural anabolic and catabolic specificities for NADH and NADPH complicate in vitro biotransformations. Synthetic biochemistry systems have often dealt with these problems by careful considerations of cofactor stoichiometry in pathway design, through the use of expensive sacrificial metabolites, re-engineering enzymes so that only a single cofactor type is needed, adding excess cofactors, or constantly adding intermediates to the reaction mix to sustain the process.
In this work, we created a robust node of control to balance the production and consumption of NADPH and NADH in a self-regulating and self-balancing manner. To our knowledge, this is the first in vitro pathway that maintains cofactor balance without requiring adherence to stoichiometry in the generation and utilization of cofactors to ensure carbon flux. In part because the system can sustain high levels of NADPH, we can drive the transformation to near completion, converting pyruvate to either PHB or isoprene at nearly 100% of the theoretical yield. Moreover, the high yields in our system are robust to 10-fold variations in cofactor levels.
Ultimately it will be necessary to expand this pathway to incorporate the conversion of low-cost substrates such as glucose or other sugars into pyruvate, which would involve the glycolysis pathway or parts of the glycolysis pathway. Indeed a synthetic biochemistry system employing glycolysis has been demonstrated previously5. Building more complex compounds from acetyl-CoA such as fatty acids, polyketides and other isoprenoids will also require the incorporation and recycling of ATP. Thus, it would be desirable to develop simple methods to regulate the ATP/ADP levels within the context of synthetic biochemistry. ATP regulation will be an interesting challenge for future synthetic biochemistry pathway designs. Ultimately, developing regulatory systems similar to the purge valve employed here will free synthetic biochemistry system design from having to consume the high-energy cofactors during the anabolic phase in perfect stoichiometric balance. Thus, our approach can help diversify the chemical targets of synthetic biochemistry.
Miller Luria-Bertani (LB) media or Miller LB-agar (BD Difco) was used for growth of bacterial strains in liquid or solid media. E. coli BL21-Gold(DE3) (B, F-, ompT, hsdSB, (rB−,mB−), dcm+,Tetr,galλ, (DE3) endA Hte) (Agilent) was used as a host for both cloning and expression of recombinant proteins using pET vectors. E. coli TOP10(DE3) (F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ−) was used for expression of recombinant proteins from the pBAD/p15A vector37. Plasmids pET28a(+) and pET22b(+) were purchased from Novagen. HotStart Taq Mastermix (Denville) was used for gene amplification from genomic or plasmid DNA. Phusion DNA polymerase (Finnizymes), Taq DNA ligase (MCLab), and T5 Exonuclease (Epicenter) were purchased separately and used to make the assembly master mix (AMM) used for cloning (ref). ATP, (±)-α-lipoic acid, pyruvate, coenzyme A and NAD+ were from Sigma.
The expression plasmids for the PHB enzymes were constructed from the pET28a plasmid backbone using the Nde1- and Sac1-cut sites to produce constructs with an N-terminal 6 × His tag for purification. The genes encoding acetyl-CoA acetyltraferase (phaA; YP_725941) and acetoacetyl-CoA reductase (phaB; YP_725942)23 were amplified and cloned from Ralstonia eutropha genomic DNA. The gene encoding PHB synthase38,39 (phaC; HE_610111) was synthesized and codon optimized for expression in an E. coli host at Life Technologies before being subcloned into the pET28a expression vector. For the isoprene pathway, the constructs were the same as described in reference30.
E. coli BL21-Gold cells were used as the host strain for enzyme expression. All enzymes were expressed in LB media supplemented with 50 μg ml−1 kanamycin and were induced with 0.2 mM isopropyl-β-D-1-thiogalactopyranoside added to the culture at the end of log phase growth. The phaA, phaB, MVK, PMVK and IspS were induced at 37 °C overnight and phaB, THL/HMGR, HMGS and IDI were induced at 18 °C overnight. The phaC was induced at 25 °C for 5 h before harvesting.
Cells from 0.5 l of culture were harvested by centrifugation and resuspended in 150 mM Tris pH 7.5, 100 mM NaCl. The cells were lysed on ice with sonication and the cell debris was removed by 12,000 g centrifugation at 4 °C. The supernatant was then mixed with 5 ml nickel-nitrilotriacetic acid agarose and after 30 min, the agarose slurry was loaded onto a gravity column and washed with five-column volumes of 100 mM Tris pH 7.5, 100 mM NaCl and 15 mM imidazole. The enzyme was then eluted with 250 mM imidazole, 100 mM Tris pH 7.5. The resulting enzyme was dialysed into 50 mM Tris pH 7.5, 50 mM NaCl and stored at 4 °C.
Expression vectors for PDH subunits and E. coli LplA
The E1, E2 and E3 domains were all amplified separately from G. stearothermophilus genomic DNA (ATCC) using primers that contained 15–20 bp complementary to the gene and 15–20 bp complementary to the multiple cloning site in the vector where the gene would be placed. The genes encoding E1α and E1β were amplified together from G. stearothermophilus genomic DNA and cloned into pET28a(+) that had been digested with NcoI and XhoI. This created a tagless construct for E1 expression under control of the T7 promoter where E1α translation uses the ribosomal binding site from the pET28 vector while E1β uses the endogenous ribosomal binding site from G. stearothermophilus. The E2 and E3 domains were amplified separately and cloned into pET22b(+) digested with NdeI and XhoI or pET28a(+) digested with NcoI and XhoI, respectively, to create tagless E2 and E3 constructs. The E. coli lipoate protein ligase, LplA, was amplified from E. coli K12 genomic DNA and assembled into pBAD/p15A37 digested with XhoI and EcoRI to create 6 × His-LplA.
Overexpression and purification of PDH subunits and LplA
All E. coli strains were grown at 37 °C in LB-media supplemented with appropriate antibiotic (100 μg ml−1 ampicillin, 50 μg ml−1 kanamycin or 34 μg ml−1 chloramphenicol). For all constructs, 5 ml of overnight starter culture was used to inoculate 1 l of LB-media. Once the OD600 reached 0.6, 0.3 mM IPTG (pET vectors) or 0.02% arabinose (pBAD/p15A) was added to induce protein expression. After 16 h, cells were harvested, resuspended (4 ml g−1 wet cells) in 50 mM Tris-Cl pH 7.5, 0.1 M NaCl (buffer A), lysed by sonication and cell debris removed by centrifugation at 30,000 g for 20 min.
E. coli lysate (25 ml) containing 6 × His-LplA was loaded onto a 3-ml nickel-nitrilotriacetic acid resin (Qiagen), washed with 25 ml buffer A containing 10 mM imidazole and eluted with 5 ml buffer A containing 250 mM imidazole. Pure 6 × His-LplA was then stored at 4 °C until use.
The individual domains of G. stearothermophilus PDH were partially purified from E. coli lysates by heat before modification and reconstitution of the PDH complex. E1αβ-, E2- or E3-containing lysates were incubated at 65 °C for 35 min to heat denature E. coli proteins followed by centrifugation at 30,000g for 20 min to pellet the precipitated proteins. Nearly all of the PDH domains remain in the supernatant. Next, the E2 domain was lipoated in the heated extract by the addition of 1 mM (±)-α-lipoic acid, 2 mM ATP, 3 mM MgCl2 and 50 μg of purified 6 × His-LplA. The lipoation reaction was then allowed to proceed with gentle mixing overnight at 25 °C, yielding lipoated E2 (E2lip). After lipoation, E1αβ, E2lip and E3 were mixed in a 3:1:3 molar ratio and incubated for at least 1 h at 25 °C to form the active GsPDH complex. The GsPDH complex was then isolated by ultracentrifugation (Beckman) for 4 h at 95,000 g. The resulting yellow pellet was resuspended in 20 mM Tris-Cl, pH 7.5 in 1/50 the starting volume and assayed for activity33,34,40. SDS–polyacrylamide gel electrophoresis analysis confirmed the presence of all four domains and indicated that the preparation was >90% pure. The reconstituted complex was stored at 4 °C until use.
Enzyme activity and optimization
NoxE was assayed by monitoring the oxidation of NAD(P)H at 340 nm. The assay was carried out in 100 mM Tris–HCl pH 7.5, 5 mM MgCl2, 5 mM KCl and 0.2 mM NAD(P)H.
WT and mutant PDH were assayed by monitoring the reduction of NAD(P)+ at 340 nm. The assay was carried out in 50 mM Tris pH 7.5, 5 mM MgCl2, 5 mM pyruvate, 1 mM CoA and 0.5 mM of NAD(P)+.
PhaC was assayed by monitoring the absorbance of the hydrolysis of the thioester bond of the substrate 3HBCoA at 235 nm. The assay was carried out in 100 mM Tris pH 7.5, 5 mM MgCl2 and 0.15 mM 3HBCoA.
Activity of NADPH-consuming isoprene pathway enzymes were monitored with the decrease of absorbance at 340 nm. Activity of ATP-consuming enzymes were monitored with a coupled assay system with pyruvate kinase and lactate dehydrogenase at 340 nm. The amount of each enzyme in the reconstituted isoprene pathway described below is show in Supplementary Table 2.
Final PHB reaction conditions and analysis
The optimized self-sustaining reaction for the biotransformation of pyruvate to PHB was composed of 250 mM Tris pH 7.5, 5 mM MgCl, 5 mM KCl, 0.5 mM CoA, 0.1 mM NAD+, 0.5 mM NADP+, 50 mM pyruvate, 2 μg PDHNADH, 76 μg PDHNADPH, 23 μg phaA, 14 μgphaB and 32 μg phaC in a final reaction volume of 200 μl. The reactions were initiated with the addition of pyruvate, which was left out of the initial mixture. All PHB reactions were performed at room temperature. For testing the autoregulatory behaviour of the purge valve, some enzyme concentrations were sub-optimal: 5 μg phaA, 2.5 μg phaB and 1.9 μg phaC.
To assay for PHB, the reactions were lyophilized and incubated with 1 ml chloroform, 0.45 ml methanol and 0.05 ml H2SO4 to hydrolyse the polymer and generate methyl 3-hydroxybutyrate. The reactions were extracted with 0.5 ml water and 1 μl of the chloroform layer was applied to a 0.25-micron HP-Innowax column using a HP 5890 Series II gas chromatogram. The gas chromatography method used an injection temperature that was held at 35 °C for 5 min before it was increased to 275 °C over 40 min. The peak intensities were compared with an authentic standard to assess concentrations.
Isoprene reaction conditions and analysis
In vitro production of isoprene was performed as described previously30 with the following changes. 200 μl reactions were set up in 2-ml gas-tight vials containing enzymes, 3 mM pyruvate, 15 mM ATP, 0.6 mM CoA, 0.2 mM NAD+, 0.4 mM NADP+ (or 5 mM NADPH), 10 mM MgCl2, 20 mM KCl and 0.1 mM thiamine pyrophosphate in 100 mM Tris-Cl pH 8.5 and incubated at 32 °C for 18 h. Isoprene production was monitored by direct sampling of 100 μl of the headspace using a 100-μl gas-tight syringe. The headspace was analysed by GC-FID (HP5980II) equipped with a GS-GasPro column (0.32 mm × 30 m, Agilent) as described previously30. The carrier gas was helium with a flow rate of 5 ml min−1. The oven temperature was kept at 120 °C for 3 min then raised to 230 °C at 5 C min−1 then to 250 °C at 20 °C min−1 and held for at 250 °C for 9 min. The inlet and detector temperatures were kept at 270 and 330 °C, respectively. The amount of isoprene produced was quantified by comparison with a standard curve of various isoprene concentrations sampled in the same manner.
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We thank members of the Bowie lab for critical reading of this manuscript. The work was supported by the DOE grant DE-FC02-02ER63421 to J.U.B.
The authors declare no competing financial interests.
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Opgenorth, P., Korman, T. & Bowie, J. A synthetic biochemistry molecular purge valve module that maintains redox balance. Nat Commun 5, 4113 (2014). https://doi.org/10.1038/ncomms5113
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