Figure 6 : H3K36 methylation and acetylation are cell cycle regulated.

From: A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice

Figure 6

(a) Flow cytometry analysis of wild-type cells following release from nitrogen starvation. The data shown were performed from at least two biological replicates. (b) Acid-extracted histones prepared from a wild-type strain were resolved on a 4–20% SDS-PAGE gel, transferred to a PVDF membrane and probed for H3K36me3, H3K36me1 and H3K36ac. α-H3 is shown as a loading control. Western blot analysis of H3K36me3, H3K36me2, H3K36me1 and H3K36Ac in wild-type nuclear extract at the times shown following release from nitrogen starvation.